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Merck

06-852

抗-Spinophilin/Neurabin-II抗体

Upstate®, from rabbit

同義詞:

Protein phosphatase 1 regulatory subunit 9B 3 neurabin II, protein phosphatase 1, regulatory (inhibitor) subunit 9B, protein phosphatase 1, regulatory subunit 9B, protein phosphatase 1, regulatory subunit 9B, spinophilin

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分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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生物源

rabbit

品質等級

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

rat

製造商/商標名

Upstate®

技術

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

human ... PPP1R9B(84687)

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1 of 4

本產品
ABC61-MN503707-1221
antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

species reactivity

rat

species reactivity

human, mouse

species reactivity

rat, mouse, canine

species reactivity

rat, human, mouse, bovine

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

UniProt accession no.

Q96SB3

UniProt accession no.

Q12972

UniProt accession no.

O35274

UniProt accession no.

Q15257

technique(s)

immunocytochemistry: suitable, western blot: suitable, immunoprecipitation (IP): suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunoprecipitation (IP): 10-20 μg using rat brain extract (S1 fraction), indirect immunofluorescence: 10-20 using MDCK cells., microarray: suitable, western blot: 1-2 μg/mL using mouse and rat brain extracts (S1 fraction)

technique(s)

western blot: suitable

一般說明

120 kDa
Spinophilin/neurabin II是一种肌动蛋白相关的支架蛋白,富含神经元的树突棘。

免疫原

GST融合蛋白,对应人脑Spinophilin/Neurabin-II的286-390位残基。

應用

免疫沉淀:
4 μg的先前批次对来自500 μg大鼠脑微粒体制剂(目录号12-144)的Spinophilin/Neurabin-II进行免疫沉淀。
抗Spinophilin/Neurabin-II抗体是一种针对Spinophilin/Neurabin-II的抗体,可用于IC、IP & WB。
研究子类别
突触 & 突触生物学

神经元 & 胶质标记
研究类别
神经科学

生化/生理作用

其他物种未知。
特异于spinophilin,Mr 120 kDa。也可识别疑似为spinophilin(Mr 95kDa)裂解产物的条带。

外觀

形式:纯化
纯化的兔多克隆IgG,溶于含有70%储存缓冲液(0.014 M 磷酸盐缓冲液(pH 7.6)、0.175 M NaCl、0.07%叠氮化钠)和30%甘油的缓冲液中。储存于 -20°C。
蛋白G纯化

準備報告

自收到之日起在-20ºC可稳定保存1年。

处理建议:
收到后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。分装到微量离心管中,并储存于 -20°C。避免反复冻融,其可能会破坏IgG并影响产品性能。注意:冷藏室温度变化至低于-20°C时可能导致含甘油的溶液在储存过程中冻结。

分析報告

对照
脑组织
已通过蛋白免疫印迹对大鼠脑微粒体制剂进行了常规评估。

蛋白质印迹分析:
0.25-1 μg/mL该批次产品已在20 μg大鼠脑微粒体制剂(目录号12-144)中检测到Spinophilin/Neurabin-II。

其他說明

浓度:请参考批次特异性浓缩物的分析证书。

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Age-related changes in Usp9x protein expression and DNA methylation in mouse brain.
Jun Xu
Brain Research Molecular Brain Research null
Ryan T Terry-Lorenzo et al.
The Journal of biological chemistry, 277(48), 46535-46543 (2002-09-25)
Inhibitor-2 (I-2) bound protein phosphatase-1 (PP1) and several PP1-binding proteins from rat brain extracts, including the actin-binding proteins, neurabin I and neurabin II. Neurabins from rat brain lysates were sedimented by I-2 and its structural homologue, I-4. The central domain
A Satoh et al.
The Journal of biological chemistry, 273(6), 3470-3475 (1998-03-07)
In a preceding paper, we reported a novel actin filament (F-actin)-binding protein, named neurabin, which was specifically expressed in neural tissue and implicated in neurite formation. We purified from rat brain another F-actin-binding protein, which had a domain organization similar
Bridget M Nugent et al.
Nature neuroscience, 18(5), 690-697 (2015-03-31)
The developing mammalian brain is destined for a female phenotype unless exposed to gonadal hormones during a perinatal sensitive period. It has been assumed that the undifferentiated brain is masculinized by direct induction of transcription by ligand-activated nuclear steroid receptors.
Interaction of rat lin-10 with brain-enriched F-actin-binding protein, neurabin-II/spinophilin.
Ide, N, et al.
Biochemical and biophysical research communications, 244, 258-262 (1998)

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