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Merck

09-403

抗-TKS5 (SH3 #1) 抗体

from rabbit

同義詞:

Adaptor protein TKS5, Five SH3 domain-containing protein, SH3 and PX domains 2A, SH3 multiple domains 1, SH3 multiple domains protein 1, five SH3 domains

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關於此項目

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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生物源

rabbit

品質等級

共軛

unconjugated

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

mouse, human

技術

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... SH3PXD2A(9644)
mouse ... Sh3Pxd2A(14218)

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Show Differences

1 of 4

本產品
09-268MABT336MABT337
clone

polyclonal

clone

polyclonal

clone

13H6.3, monoclonal

clone

9H2.1, monoclonal

species reactivity

mouse, human

species reactivity

mouse, human

species reactivity

human

species reactivity

human

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

mouse

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

UniProt accession no.

O89032

UniProt accession no.

O89032

UniProt accession no.

Q5TCZ1

UniProt accession no.

Q5TCZ1

Gene Information

human ... SH3PXD2A(9644)
mouse ... Sh3Pxd2A(14218)

Gene Information

human ... SH3PXD2A(9644)

Gene Information

human ... SH3PXD2A(9644)

Gene Information

human ... SH3PXD2A(9644)

一般說明

130-150 kDa 某些细胞裂解液中可能会出现未表征的条带。
Tks5(以前称为Fish)是一种大型支架蛋白,具有一个氨基末端PX结构域和五个SH3结构域。它在正常成纤维细胞中是细胞质的。在Src转化的细胞中,Tks5定位于足小体。Tks5和组成型活性Src在上皮癌细胞中的共表达导致足小体/侵袭性伪足的出现。Tks5表达降低的细胞通过基质胶的侵袭性很差。Tks5在一些侵袭性人类癌细胞系和肿瘤组织中表达并定位于足小体/侵袭性伪足,特别是乳腺癌和黑色素瘤。因此,Tks5似乎是足小体/侵袭性伪足形成和某些癌细胞侵袭所必需的。

免疫原

表位:166-225 aa
重组蛋白,aa:166-225。同源性:人、犬、马、大鼠、牛(100%,60/60);鸡(93%,56/60)

應用

抗TKS5 (SH3 #1)抗体可检测TKS5 (SH3 #1) 水平 & 已出版&并经过验证可用于WB,IC & IP。
研究子类别
细胞骨架信号转导
研究类别
细胞结构
蛋白质印迹分析:抗体可在20 μg NIH/3T3(100%融合)裂解液中检测到TKS5。

生化/生理作用

其他同源性:人、犬、马、大鼠和牛(100%序列同源性),鸡(93%序列同源性)

外觀

在含0.05%NaN3的0.1M Tris-甘氨酸(pH7.4)150mM NaCl中的纯化抗体。
形式:纯化
蛋白A纯化

準備報告

自收到之日起,在2-8°C以未稀释的等分试样可保存1年。

分析報告

对照
蛋白质印迹:
NIH/3T3(100%融合)裂解液
免疫细胞化学:
小鼠3T3-Src(Y527F)细胞
通过蛋白质印迹在NIH/3T3(100%融合)裂解液中进行了常规评估。

其他說明

浓度:请参考批次特异性浓缩物的分析证书。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Samuel J Ghilardi et al.
Molecular biology of the cell, 32(18), 1707-1723 (2021-07-01)
Interactions between the actin cytoskeleton and the plasma membrane are important in many eukaryotic cellular processes. During these processes, actin structures deform the cell membrane outward by applying forces parallel to the fiber's major axis (as in migration) or they
Karla C Williams et al.
Oncogene, 38(19), 3598-3615 (2019-01-18)
Invadopodia are cell protrusions that mediate cancer cell extravasation but the microenvironmental cues and signaling factors that induce invadopodia formation during extravasation remain unclear. Using intravital imaging and loss of function experiments, we determined invadopodia contain receptors involved in chemotaxis
Nawal Bendris et al.
Journal of cell science, 129(14), 2804-2816 (2016-06-10)
The ability of cancer cells to degrade the extracellular matrix and invade interstitial tissues contributes to their metastatic potential. We recently showed that overexpression of sorting nexin 9 (SNX9) leads to increased cell invasion and metastasis in animal models, which
Deciphering the involvement of the Hippo pathway co-regulators, YAP/TAZ in invadopodia formation and matrix degradation.
Venghateri, et al.
Cell Death & Disease, 14, 290-290 (2023)
Sara K Donnelly et al.
The Journal of cell biology, 216(12), 4331-4349 (2017-10-25)
The initial step of metastasis is the local invasion of tumor cells into the surrounding tissue. Invadopodia are actin-based protrusions that mediate the matrix degradation necessary for invasion and metastasis of tumor cells. We demonstrate that Rac3 GTPase is critical

相關內容

QCM™ Gelatin Invadopodia Assays provide optimized materials and protocols to enable reproducible analysis of invadopodia in invasive tumor cells (Catalog No. ECM670 for green fluorescence, Catalogue No. ECM671 for red fluorescence). Reagents are provided for coating glass culture surfaces with fluorescent matrix and for colocalizing the actin cytoskeleton and nuclei with invadopodial degradation sites. This assay may also be used for assessing the activity of inhibitors and promoters of invadopodia formation and function. Furthermore, different cell types and individual cells in heterogeneous populations may be analyzed for invasive potential. Finally, the assay kits provide troubleshooting suggestions, recommendations for coating on multiple substrate formats, and example studies in several assay systems (e.g., various cell types, time-course studies, degradation modulation).

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