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Merck

1.02129

Chromolith® RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 100 mm x 4.6 mm HPLC Column

同義詞:

Chromolith® RP-18封端

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分類程式碼代碼:
41115709
NACRES:
SB.52

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產品名稱

Chromolith® Performance RP-18封端100-4.6 HPLC柱, L × I.D. 100 mm x 4.6 mm HPLC column

材料

PEEK column

品質等級

agency

suitable for USP L1

產品線

Chromolith®

特點

endcapped

包裝

1 ea of

標籤範圍

18% Carbon loading

參數

200 bar pressure
45 °C max. temp.

技術

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

柱長度 × 內徑

10 cm × 4.6 mm

基質

monolithic silica

基質活性組

C18 bonding phase

孔徑

13 nm mesoporosity
2 μm macroporosity

工作pH值範圍

2-7.5

分離技術

reversed phase

儲存溫度

no temp limit

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1 of 4

本產品
1.520061.520011.51450
matrix active group

C18 bonding phase

matrix active group

C18 bonding phase

matrix active group

C18 bonding phase

matrix active group

C18 bonding phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

pore size

13 nm mesoporosity, 2 μm macroporosity

pore size

1.5 μm macroporosity, 13 nm mesoporosity

pore size

13 nm mesoporosity, 2 μm macroporosity

pore size

13 nm mesoporosity, 2 μm macroporosity

technique(s)

HPLC: suitable, mass spectrometry (MS): suitable (High), LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

matrix

monolithic silica

matrix

monolithic silica

matrix

monolithic silica

matrix

monolithic silica

一般說明

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® Performance RP-18 endcapped HPLC columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å) and the surface modification is octadecylsilane with full end-capping.

Chromolith® Performance RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column.

Chromolith®Performance RP-18 endcapped columns are perfectly suitable for LC-MS applications. The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:

  • Rapid separations at very low column back-pressure
  • High matrix tolerance, allowing time and cost savings with simplified workflows
  • Cost savings due to extended column lifetimes
  • Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are: Chromolith® RP-18 endcapped 10-4.6 guard cartridges (3 pieces) [1.51452] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032] Or Chromolith® RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.51451] with the Chromolith® Guard Cartridge Holder 10 mm x 4.6 mm [1.52033] The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467] Need more efficiency? Chromolith®HighResolution RP-18 endcapped 100-4.6 HPLC columns [1.52022] provide a column efficiency exceeding 140,000 plates/meter. Learn more: Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments. Supelco HPLC Products: The Best Choice for Any LC Instrument

應用


  • 盐污染降低了实验春池群落中的浊度、溶解有机物和蓝藻:讨论了氯化钙的环境应用,重点讨论了氯化钙在受盐污染影响生态系统管理中的应用,展示了其在环境修复和管理中的作用。(Vigil and Schuler, 2024).



分析報告

外观:符合
碳含量:17,0 - 18,6 %
压力: ≤ 30 bar
稳定性测试:符合
性能(蒽)
理论塔板数(N/m)(蒽): ≥ 80000
容量系数(蒽):3,9 - 4,6
尾部(USP):0,90 - 1,60 %
选择性
分离因子(蒽/孕酮):1.95 - 2.35

法律資訊

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10-13 - German Storage Class 10 to 13


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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文章

LC-UV-MS workflow details teriparatide peptide mapping, including enzymatic digestion, separation conditions, and QTOF mass spectrometer identification.

Chromolith® RP-18e columns optimize Oligo Standard 6 separation with varied flow rates and ion-pairing reagent evaluation.

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Chromolith® RP-18e 色譜柱可藉由不同的流速和離子配對試劑評估,優化 Oligo Standard 6 的分離效果。

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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the particle size of Chromolith 18 endcap column

    1 answer
    1. This product is not comprised of distinct particles; rather it is a a monolithic silica column. This silica monolith contains both mesopores (~13 nm) and macropores (~2 um) Please see the following for more information:
      https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/chromolith-hplc-and-uhplc-columns

      Helpful?

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