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Merck

12-309

HeLa Nuclear Extract

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分類程式碼代碼:
41105330
eCl@ss:
32011807
NACRES:
NA.41

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生物源

human

品質等級

100

形狀

liquid

製造商／商標名

Upstate®

濃度

2.0 mg/mL

技術

western blot: suitable

運輸包裝

dry ice

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本產品
126-2.5NXTRACTP1167
HeLa Nuclear Extract

Sigma-Aldrich

12-309

HeLa Nuclear Extract

SAFC

SAFC

126-2.5

EHS Matrix Extract

NuCLEAR™提取试剂盒 For mammalian tissue or cultured cells

Sigma-Aldrich

NXTRACT

NuCLEAR™提取试剂盒

垂体提取物 牛 lyophilized powder, suitable for cell culture

Sigma-Aldrich

P1167

垂体提取物 牛

biological source

human

biological source

-

biological source

-

biological source

bovine

technique(s)

western blot: suitable

technique(s)

-

technique(s)

protein extraction: suitable, western blot: suitable

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

-

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

Quality Level

100

Quality Level

100

Quality Level

300

Quality Level

200

form

liquid

form

liquid

form

-

form

lyophilized powder

一般說明

Nuclear extract prepared from human HeLa cells using a modified protocol of Dignam, et al., which contains a variety of DNA binding proteins and transcription factors.

生化／生理作用

HeLa

外觀

RIPA sample buffer containing inhibitors. Frozen solution.

準備報告

Stable for 6 months at -70°C from date of shipment.

分析報告

Routinely evaluated as a positive control for many of Upstates antibodies.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H319

危險分類

Eye Irrit. 2

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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J D Dignam et al.
Nucleic acids research, 11(5), 1475-1489 (1983-03-11)
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major
Tabetha Sundin et al.
Biochimie, 94(12), 2639-2648 (2012-08-21)
Isoprenoids are recognized for their ability to suppress carcinogenic processes in vivo and in vitro. We previously established that the isoprenoid, perillyl alcohol, acted mechanistically on translation of specific proteins through modulation of mechanistic target of rapamycin (mTOR) signaling. Telomerase-the

Questions

1–2 of 2 Questions  

  1. Is this product in vitro transcription grade? What are the components provided with this item? Can I used this item to test for human rna polymerase 2 transcription?

    1 answer

    1. This product is not tested for a specific application, such as transcription efficiency. The material is supplied as a single vial of a 50 ug/25 uL solution containing RIPA buffer and inhibitors. No other components are included. Note that unless otherwise stated this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

      Please see the link below to review available Certificates of Analysis:
      https://www.emdmillipore.com/product/HeLa-Nuclear-Extract,MM_NF-12-309#anchor_COA

      Helpful?

  2. Can I use this HeLa Nuclear Extract for deadenylase assay? specifically, Does this product contain the complex of deadenylase in addition to a variety of DNA binding proteins and transcription factors?

    1 answer

    1. This product has not been tested for use in deadenylation. There is no reason to suspect that it would not be suitable. However, the product is stored in RIPA buffer. Please check the literature for potential interference of RIPA in the adenylase assay reaction.

      Helpful?

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