To prepare this product, add 500µl of ultrapure water to make a 1mg/ml stock solution. It is recommended to aliquot the solution to avoid repeated freezing and thawing. The reconstituted solution can be stored for up to 6 months at -20°C. This product is suitable for use in immunohistochemistry at a 1:1000-1:4000 dilution. You can review available Certificates of Analysis by visiting the documentation section on our website.
生物源
rabbit
品質等級
共軛
peroxidase conjugate
抗體表格
affinity purified immunoglobulin
抗體產品種類
secondary antibodies
無性繁殖
polyclonal
物種活性
sheep
製造商／商標名
Upstate®
技術
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
運輸包裝
wet ice
目標翻譯後修改
unmodified
1 of 4
本產品 | 12-349 | AP180P | AP124C |
|---|---|---|---|
| species reactivity sheep | species reactivity mouse | species reactivity goat | species reactivity mouse |
| conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate CY3 conjugate |
| biological source rabbit | biological source goat | biological source donkey | biological source goat |
| antibody form affinity purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form F(ab′)2 fragment of affinity isolated antibody |
| technique(s) ELISA: suitable, western blot: suitable, immunohistochemistry: suitable | technique(s) ELISA: suitable, immunohistochemistry: suitable, western blot: suitable | technique(s) ELISA: suitable, immunohistochemistry: suitable, western blot: suitable | technique(s) immunofluorescence: suitable |
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
一般說明
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
應用
Rabbit anti-Sheep IgG Antibody, HRP conjugate detects level of Sheep IgG & has been published & validated for use in ELISA, WB, IH.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
外觀
0.02M Potassium Phosphate, 0.15M NaCl, pH 7.2, 10mg/ml BSA, and 0.01% gentamicin sulfate
準備報告
Lyophilized: 2 years at 4°C; Rehydrated: 6 months at -20°C.
分析報告
Routinely evaluated by western blot
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Arvin Bansal et al.
Journal of proteomics, 73(10), 2041-2049 (2010-07-06)
Exercise has been shown to improve function of the left ventricle (LV) following myocardial infarction (MI). The mechanisms to explain this benefit have not been fully delineated, but may involve improved mechanics resulting in unloading effects and increased endothelial nitric
Donel A Sequea et al.
Mechanisms of ageing and development, 134(1-2), 60-63 (2012-12-19)
Moderate calorie restriction (CR) can improve insulin-stimulated Akt phosphorylation and glucose uptake in muscles from 24 month-old rats, but the specific Akt substrates linking CR-effects on Akt to glucose uptake and other cellular processes are uncertain. We probed CR's influence
Susannah L McKay et al.
PloS one, 6(1), e16077-e16077 (2011-02-02)
There is mounting evidence to suggest that the synthesis of pre-mRNA transcripts and their subsequent splicing are coordinated events. Previous studies have implicated the mammalian spliceosomal U2 snRNP as having a novel role in stimulating transcriptional elongation in vitro through
Naveen Sharma et al.
PloS one, 8(6), e65118-e65118 (2013-06-12)
Calorie restriction (CR) (consuming ~60% of ad libitum, AL, intake) improves whole body insulin sensitivity and enhances insulin-stimulated glucose uptake by isolated skeletal muscles. However, little is known about CR-effects on in vivo glucose uptake and insulin signaling in muscle.
Naveen Sharma et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 303(12), R1261-R1267 (2012-11-02)
Akt is a serine/threonine kinase that plays a key role in numerous cellular functions including metabolism, growth, protein synthesis, apoptosis, and cell proliferation. The most consistent and robust effect of moderate calorie restriction (CR; ~60% of ad libitum, AL, food
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Could you please provide the reconstitution method for this product and the recommended concentration for various uses?
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What is the method of reconstitution for this product and what is the concentration recommended for various uses?
1 answer-
This product can be prepared by adding 500µl of ultrapure water to make a 1mg/ml stock solution. Aliquot to avoid repeated freezing and thawing. The reconstituted solution may be stored up to 6 months at -20°C. This product is tested for use in immunohistochemistry, at which a 1:1000-1:4000 dilution is suitable. See the link below to review available Certificates of Analysis: https://www.emdmillipore.com/product/Rabbit-Anti-Sheep-IgG-Antibody-HRP-conjugate,MM_NF-12-342#documentation
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