14-750-M

Abl (H396P) Protein, active, 10 µg

Active, N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the H396P mutation. Expressed by baculovirus in Sf21 insect cells. For use in Kinase Assays.

• 分類程式碼代碼: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

屬性

• 生物源: human
• 品質等級: 300
• 重組細胞: expressed in baculovirus infected Sf21 cells
• 分子量: M_w 121 kDa
• 製造商／商標名: Upstate^®
• 技術: activity assay: suitable (kinase)
• UniProt登錄號: P00519; U07563

描述

一般說明

Product Source: N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the H396P mutation. Expressed by baculovirus in Sf21 insect cells.

The Abl tyrosine kinase inhibitor STI571 (Gleevec) is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse, due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the H396P mutation, which confers resistance to drug binding due to steric interference. (Corbin AS. et al, Blood, (2002); 101:4611-4614 and La Rosee P. et al, Cancer Research; 62:7149-7153).

應用

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

生化／生理作用

Protein Target: Abl

Target Sub-Family: TK

包裝

Also available in 250μg size as catalog # 14-750M - Call for pricing and availability.

外觀

Ni²⁺/NTA-agarose

50mM Tris/HCl pH7.5, 150mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution.

準備報告

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

分析報告

Routinely evaluated by phosphorylation of Abltide.

其他說明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H317
• 防範說明: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危險分類: Skin Sens. 1
• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable