生物源
mouse
品質等級
共軛
peroxidase conjugate
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
PY20, monoclonal
物種活性（以同源性預測）
all
製造商／商標名
Upstate®
技術
ELISA: suitable
western blot: suitable
同型
IgG2b
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... PID1(55022)
相關類別
1 of 4
本產品 | 16-316 | 16-105 | 16-101 |
|---|---|---|---|
| clone PY20, monoclonal | clone 4G10®, monoclonal, PY20, monoclonal | clone 4G10®, monoclonal | clone 4G10®, monoclonal |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| Gene Information human ... PID1(55022) | Gene Information human ... PID1(55022) | Gene Information human ... PID1(55022) | Gene Information human ... PID1(55022) |
| conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate - |
| antibody form purified immunoglobulin | antibody form - | antibody form purified immunoglobulin | antibody form purified antibody |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
一般說明
Protein tyrosine phosphorylation was first described in 1979. Large numbers of tyrosine kinases have been described since, and many of the signaling pathways initiated by tyrosine phosphorylation have been delineated. Availability of a top-quality anti-phosphotyrosine antibody has allowed the study of tyrosine kinase activity by simple blotting procedures, and without routine need of large quantities of radioactivity. The 4G10 monoclonal anti-phosphotyrosine antibody (Cat. # 05-321) has been widely recognized as a highly specific and sensitive. This antibody is suitable for all applications involving protein tyrosine phosphorylation. The slightly less sensitive, but still highly regarded clone PY20 can be used as an alternative to the 4G10 product line.
Varies
免疫原
Epitope: phosphotyrosine
Phosphotyrosine conjugated to carrier protein
應用
Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate is a Mouse Monoclonal Antibody for the detection of Phosphotyrosine & has been validated in WB, ELISA.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
生化／生理作用
Recognizes proteins containing phosphotyrosine residues, regardless of origin.
外觀
Protein A purified
100 μL of protein A purified mouse IgG2b in PBS containing 0.05% Kathon® and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:2000- 1:4000 in 10 mL blocking buffer. Liquid.
準備報告
6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析報告
Routinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.
法律資訊
Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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訊號詞
Warning
危險分類
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
S F Retta et al.
Molecular biology of the cell, 9(4), 715-731 (1998-05-16)
The beta1-integrin cytoplasmic domain consists of a membrane proximal subdomain common to the four known isoforms ("common" region) and a distal subdomain specific for each isoform ("variable" region). To investigate in detail the role of these subdomains in integrin-dependent cellular
Becky K Brisson et al.
PloS one, 7(9), e45588-e45588 (2012-10-03)
Insulin-like growth factor-I (IGF-I) is an essential growth factor that regulates the processes necessary for cell proliferation, differentiation, and survival. The Igf1 gene encodes mature IGF-I and a carboxy-terminal extension called the E-peptide. In rodents, alternative splicing and post-translational processing
Julia Durzyńska et al.
Endocrinology, 154(3), 1215-1224 (2013-02-15)
IGF-I is a key regulator of muscle development and growth. The pre-pro-peptide produced by the Igf1gene undergoes several posttranslational processing steps to result in a secreted mature protein, which is thought to be the obligate ligand for the IGF-I receptor
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