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本產品 | 17-10241 | 17-10111 | 17-643 |
|---|---|---|---|
| clone CMA309, monoclonal | clone polyclonal | clone polyclonal | clone polyclonal |
| antibody form purified immunoglobulin | antibody form serum | antibody form serum | antibody form serum |
| species reactivity vertebrates, human | species reactivity mouse, bovine, Saccharomyces cerevisiae, human | species reactivity Saccharomyces cerevisiae, yeast, human | species reactivity human, mouse |
| biological source mouse | biological source rabbit | biological source rabbit | biological source rabbit |
| technique(s) ChIP: suitable, western blot: suitable, immunoprecipitation (IP): suitable | technique(s) ChIP: suitable, western blot: suitable | technique(s) ChIP: suitable (ChIP-seq), dot blot: suitable, western blot: suitable | technique(s) ChIP: suitable, electrophoretic mobility shift assay: suitable, flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
一般說明
ChIPAb+乙酰基组蛋白H3（Lys27）组包括抗乙酰基组蛋白H3（Lys27）抗体，阴性对照抗体（纯化的小鼠IgG）和qPCR引物，可扩增人RPL10基因启动子内的178 bp区域。乙酰基组蛋白H3（Lys27）和阴性对照抗体以可扩展的“每个ChIP”反应大小提供，可用于功能上验证乙酰基组蛋白H3（Lys27）相关染色质的沉淀。
免疫原
應用
使用2 µg正常小鼠IgG或抗乙酰基组蛋白H3（Lys27）抗体和Magna ChIP G（目录号17-611）试剂盒，对从HeLa细胞制备的超声染色质（每IP 1 X 106细胞当量）进行染色质免疫沉淀。 使用β-珠蛋白启动子ChIP引物和RPL10启动子引物通过qPCR验证了乙酰基组蛋白H3（Lys27）相关DNA片段的成功免疫沉淀（请参见图）。 数据表示为每个IP样品相对于每个扩增子和ChIP样品的输入染色质的输入百分比。
请参阅EZ-Magna G ChIP（目录号17-408）或EZ-ChIP（目录号17-371）协议以获取实验详细信息。
蛋白质印迹分析：
未处理（泳道1）和丁酸钠处理（泳道2）HeLa细胞的酸提取物通过电泳分离，转移到PVDF膜上，并用抗乙酰基组蛋白H3（Lys27），克隆CMA309（0.1 μg/mL）探测。 使用与HRP偶联的山羊
抗小鼠二抗和化学发光检测系统对蛋白质进行可视化（请参见图）。
染色质生物学
表观遗传学&核功能
生化／生理作用
包裝
外觀
正常小鼠IgG。两个小瓶，包含25 μg纯化小鼠IgG，溶于25 μL储存缓冲液（含0.1%叠氮化钠）中。储存于-20°C。
ChIP引物，RPL10启动子。一个小瓶含有75 μL的5 μM人RPL10启动子区域特异性引物。储存于-20°C。
对于：ACC CGT CTT CGA CAG GAC T
REV: GGA ACG GAA GAC GAG AAC AG
準備報告
分析報告
包括阴性对照小鼠IgG抗体和对人RPL10启动子特异性的对照引物。
使用2 µg正常小鼠IgG或抗乙酰基组蛋白H3（Lys27）抗体和Magna ChIP G（目录号17-611）试剂盒，对从HeLa细胞制备的超声染色质（每IP 1 X 106细胞当量）进行染色质免疫沉淀。
使用ChIP引物RPL10启动子通过qPCR验证了乙酰基组蛋白H3（Lys27）相关DNA片段的成功免疫沉淀（请参见图）。
有关实验详细信息，请参阅EZ-Magna G ChIP（目录号17-409）或EZ-ChIP（目录号17-371）方案。
法律資訊
免責聲明
儲存類別代碼
10 - Combustible liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
相關內容
Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms. Epigenetic regulation allows a cell to vary its response based on its biological and environmental contexts. Epigenetic changes can effect transcriptional and post-transcriptional regulation via mechanisms such as histone modification, chromatin and nucleosome remodeling, DNA methylation, and small and non-coding RNA-mediated regulation. These mechanisms, in cooperation with transcription factors and other nucleic acid-binding proteins, regulate gene expression. Epigenetic mechanisms of gene regulation impacts diverse areas of research—from agriculture to human health. Common epigenetic assays such as chromatin immunoprecipitation (ChIP) and RNA immunoprecipitation (RIP) rely on high quality antibodies that recognize specific epigenetic modifications for accurate results. EMD Millipore offers over 100 ChIPAb+™ and RIPAb+™ validated antibody kits that are quality tested on ChIP/RIP assays and are conveniently provided with control qPCR primers and negative control antibodies to ensure first time ChIP/RIP success.
"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."
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