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Merck

309800

二氢乙锭

A cell-permeable, chemically-reduced ethidium derivative.

同義詞:

二氢乙锭

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關於此項目

經驗公式(希爾表示法):
C21H21N3
CAS 編號:
分子量::
315.41
MDL號碼:
分類程式碼代碼:
12161505
NACRES:
NA.25

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品質等級

描述

RTECS - SF7935000

化驗

≥95% (HPLC)

形狀

solid

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
protect from light

顏色

pink

溶解度

DMSO: 20 mg/mL
DMF: soluble

運輸包裝

ambient

儲存溫度

−20°C

SMILES 字串

Nc1c2c(cnc1)c3c(cc(cc3)N)C(C2CC)c4ccccc4

InChI

1S/C21H21N3/c1-2-15-20(13-6-4-3-5-7-13)17-10-14(22)8-9-16(17)18-11-24-12-19(23)21(15)18/h3-12,15,20H,2,22-23H2,1H3

InChI 密鑰

ZDWPSSBKIMIPMY-UHFFFAOYSA-N

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本產品
37291D7008309825
color

pink

color

-

color

pink to very dark red

color

pink-white

form

solid

form

solid

form

powder

form

solid

solubility

DMSO: 20 mg/mL, DMF: soluble

solubility

acetonitrile: soluble, methanol: soluble

solubility

chloroform: 20 mg/mL

solubility

DMSO: 5 mg/mL

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

assay

≥95% (HPLC)

assay

≥95% (HPCE)

assay

≥95%

assay

≥95% (HPLC)

一般說明

细胞可渗透的化学还原性乙啶衍生物。常用于分析吞噬细胞的呼吸爆发。用于检测活性氧(ROS)的有效荧光探针。它可能通过线粒体氧化磷酸化的解偶联而在静息的白细胞中发生明显的氧化。胞质二氢乙锭显示出蓝色荧光,而该探针氧化为乙锭后,可插入细胞’DNA中,并可将细胞核染为明亮的红色荧光。
细胞可渗透的化学还原性乙锭衍生物,可用作检测活性氧(ROS)的荧光探针。它可能通过线粒体氧化磷酸化的解偶联而在静息的白细胞中发生明显的氧化。胞质二氢乙啶显示出蓝色荧光,但经氧化为乙啶并随后嵌入DNA后,可获得亮红色荧光。其常用于通过流式细胞仪对细胞中的超氧阴离子水平进行确定。

生化/生理作用

主要靶标
用于检测活性氧(ROS)的有效荧光探针
产物不与ATP竞争。
可逆:否
细胞可渗透性:具有

包裝

用惰性气体包装

準備報告

重溶后,等分,并用惰性气体冲洗并冷冻(-20°C)。储备溶液在-20°C下可稳定保存至多6个月。

其他說明

Haugen, T.S., et al. 1999.Eur. Respir.J.14, 1100.
Mancini, M., et al. 1997.J. Cell Biol.138, 449.
Carter, W.O., et al. 1994.J. Leukoc.Biol.55, 253.
Cinco, M., et al. 1994.FEMS Microbiol.Lett.122, 187.
Perticarari, S., et al. 1994.J. Immunol. Methods 170, 117.
Perticarari, S., et al. 1991.Cytometry 12, 687.
Bucana, C.D., et al. 1990.Exp.Cell Res.190, 69.
Rothe, G., and Valet, G. 1990.J. Leukoc.Biol.47, 440.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

毒性:刺激性(B)

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3


分析證明 (COA)

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Questions

  1. I noticed that a compound from your catalogue is described as chemically reduced. I was wondering what the reducing agent used for this is?

    1 answer
    1. The reduction reagent used for this compound was sodium borohydride. The exact protocol used is proprietary to the lab and has not been disclosed, but the following publication may be of interest as a reference: Thomas G, Roques B., FEBS Lett. 1972; 26:169–175. The publication describes the process as follows: ...under nitrogen, in the dark and at 0°C, 500 mg of EB in 10 ml absolute methanol were added to 220 mg NaBH4. After 24 hr stirring, methanol was removed by evaporation in vacuum and 15 ml of 3% sodium hydroxide solution were added. After several extractions with ether, the phases were washed with water and dried over anhydrous sodium sulfate. Solvent was evaporated. The precipitate was recrystallized in ether to yield a light brown powder.

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