GAPDH (46-667MAG) is functional only in Assay Buffer 1 when used at significantly lower dilutions, approximately 1 microgram of total protein per well. This specificity is the reason it is designated for use with Buffer 2 only. Most panels necessitate higher protein concentrations, making it impractical to multiplex GAPDH with panels that utilize Assay Buffer 1. When conducting assays with Assay Buffer 1, B-Tubulin MAPMate (46-713MAG) is the recommended alternative.
48-621MAG
MILLIPLEX® DNA Damage/Genotoxicity Magnetic Bead Panel - Cell Signaling Multiplex Assay
同義詞:
Cell Signaling DNA Damage Kit, DNA Damage Bead Panel, Genotoxicity Magnetic Beads
跳轉至
物種活性
mouse, human, rat
品質等級
製造商／商標名
Milliplex®
技術
multiplexing: suitable
檢測方法
fluorometric (Luminex® xMAP® technology)
儲存溫度
2-8°C
1 of 4
本產品 | 48-690MAG | 46-667MAG | MCD8MAG-48K |
|---|---|---|---|
| species reactivity mouse, human, rat | species reactivity human | species reactivity human | species reactivity mouse |
| manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. - |
| detection method fluorometric (Luminex® xMAP®) | detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex xMAP) |
一般說明
The MILLIPLEX® MAP 7-plex DNA Damage/Genotoxicity Magnetic Bead Kit (Cat. No. 48-621MAG) is a premixed kit to be used for the simultaneous quantification of the following 7 analytes in cell lysates:• ATR (total)• Chk1 (Ser345)• Chk2 (Thr68)• H2A.X (Ser139)• MDM2 (total)• p21 (total)• p53 (Ser15)
應用
Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.An overnight (4°C) incubation is recommended for best results.This assay requires 25 μL diluted cell lysate per well.This kit must be run using Assay Buffer 1 (provided).The suggested working range of protein concentration for the assay is 1 to 25 μg of total protein/well (25 μL/well at 40 to 1,000 μg/mL).Analytes available:ATR (Total);Chk1 (Ser345);Chk2 (Thr68);H2A.X (Ser139);MDM2 (total);p21 (Total);p53 (Ser15)
生化／生理作用
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
準備報告
Recommended storage for kit components is 2 - 8°C.
法律資訊
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
訊號詞
Danger
危險分類
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
儲存類別代碼
10 - Combustible liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
相關內容
Exploring genotoxicity and DNA damage through multiplexing with MILLIPLEX® multiplex genotoxicity assays using Luminex® xMAP® technology enables the high-throughput measurement of phosphorylation levels of multiple proteins simultaneously and reduces sample volume, time, and cost.
Multiplex toxicity assays enable researchers to simultaneously measure multiple toxicity biomarkers of various organs to gain a better understanding of toxicity. Explore how MILLIPLEX® multiplex toxicity assays are advancing research on liver injury, kidney toxicity, genotoxicity, and more.
Learn how multiplexing with MILLIPLEX® multiplex assays can help simplify cancer research by measuring multiple circulating biomarkers at once. Find multiplex panels to analyze JAK-STAT, mTOR, MAPK, SAPK, TGFβ signaling pathways, and more.
MILLIPLEX®細胞訊號傳導分析儀有助於檢測細胞通路中的磷酸化蛋白和總蛋白。
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Is it possible to incorporate GAPDH as a loading control into the DNA Damage panel (48-621MAG) even though it specifies the use of Assay Buffer 1 and the GAPDH MAPmate (46-667MAG) requires Assay Buffer 2? Does the difference in assay buffer formulations affect the compatibility of adding GAPDH to the panel?
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