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本產品 | 70584-M | 70921 | 71186 |
|---|---|---|---|
| form liquid | form liquid | form liquid | form liquid |
| manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® |
| storage condition OK to freeze | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze |
| shipped in wet ice | shipped in - | shipped in ambient | shipped in wet ice |
| storage temp. 2-8°C | storage temp. 10-30°C | storage temp. 10-30°C | storage temp. - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level - |
一般說明
應用
- 在离心进行蛋白质表达和免疫印迹后裂解大肠杆菌细胞颗粒
- 从细菌细胞颗粒中提取蛋白质
- 裂解大肠杆菌细胞颗粒，用于表达和纯化dMIC60（线粒体内膜蛋白）蛋白
特點和優勢
- 快速蛋白质提取和核酸降解
- 非常适合处理不同体积的各种样品
- 添加r溶菌酶溶液可确保提高提取率
法律資訊
免責聲明
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
文章
引文聚焦：BugBuster® 蛋白提取試劑可從細菌病原體中高效提取蛋白質
The combination of BugBuster® and Lysonase™ reagents greatly enhances the release of soluble proteins from Gram-positive bacteria.
Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit
Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens
相關內容
Traditionally, protein purification from E. coli consists of four distinct phases: harvest, bacterial cell lysis, lysate clarification and protein purification. Bacterial lysis typically requires several time-consuming, hands-on steps, such as freeze/thaw cycles and sonication. These harsh lysis techniques may negatively impact protein quality and contribute to sample-to-sample variability. To maintain protein activity and integrity, detergent-based lysis buffers are routinely used to avoid mechanical protein extraction methods. Regardless of the lysis method used, centrifugation is traditionally required to pellet unwanted cell debris and permit recovery of the clarified lysate. The final step, purification, is frequently performed using affinity media specific for expressed epitope tags. Agarose-based media have typically been used, either as a slurry in microcentrifuge tubes or packed into gravity-driven or spin columns. While easier to manipulate, columns are greatly affected by lysate consistency and carryover of cell debris, which can lead to clogging of the column frits.
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