跳轉至內容
Merck

71206

Benzonase® Nuclease HC

Purity > 99%, Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions

同義詞:

内切核酸酶 来源于粘质沙雷氏菌

Slide 1 of 1
登入 查看組織和合約定價

選擇尺寸

暫時無法取得訂價和供貨情況

關於此項目

CAS 編號:
9025-65-4
EC號碼:
3.1.30.2 (BRENDA, IUBMB)
MDL號碼:
MFCD00131010
分類程式碼代碼:
12352202
NACRES:
NA.54

重要文件

檢視所有文件

跳轉至

技術服務
需要援助？我們經驗豐富的科學家團隊隨時樂意為您提供支援。
讓我們為您提供幫助

產品名稱

Benzonase® 核酸酶HC，纯度> 99%, Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions

生物源

Serratia marcescens

品質等級

100

重組細胞

expressed in E. coli

化驗

>99% (SDS-PAGE)

形狀

buffered aqueous glycerol solution

製造商／商標名

Novagen®

儲存條件

OK to freeze

濃度

≥250 units/μL

雜質

<0.25 EU/kU Total endotoxin

應用

research use

運輸包裝

wet ice

儲存溫度

−20°C

尋找類似的產品？ 前往 產品比較指南

相關類別

比較類似項目

查看完整比較

Show Differences

1 of 4

本產品
71205-M70746E8263
Benzonase&#174; 核酸酶HC，纯度&gt; 99% Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions

Millipore

71206

Benzonase® 核酸酶HC，纯度> 99%

Benzonase&#174; 核酸酶HC，纯度&gt; 90%

Millipore

71205-M

Benzonase® 核酸酶HC，纯度> 90%

Benzonase®核酸酶，纯度&gt; 90%

Millipore

70746

Benzonase®核酸酶，纯度> 90%

Benzonase&#174;核酸酶，超纯 &#8805;250&#160;units/&#956;L, &#8805;99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade

Sigma-Aldrich

E8263

Benzonase®核酸酶，超纯

assay

>99% (SDS-PAGE)

assay

>90% (SDS-PAGE)

assay

>90% (SDS-PAGE)

assay

≥99% (SDS-PAGE)

biological source

Serratia marcescens

biological source

Serratia marcescens

biological source

Serratia marcescens

biological source

Serratia marcescens

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

application(s)

research use

application(s)

research use

application(s)

research use

application(s)

research use

concentration

≥250 units/μL

concentration

≥250 units/μL

concentration

25-29 units/μL

concentration

≥250 units/μL

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

一般說明

Benzonase® 核酸酶是一种用基因工程方法从粘质沙雷氏菌获得的核酸内切酶。它降解所有形式的DNA和RNA（单链、双链、线性和环状），而没有蛋白水解活性。它能够在较大范围的条件下工作，并且具有极高的比活度。该酶将核酸完全消化为长度为2至5个碱基（低于杂交极限）的5′-一磷酸酯化的寡核苷酸，非常适合从重组蛋白中去除核酸，从而符合FDA关于核酸污染的指南。Benzonase迅速水解核酸的能力使该酶成为降低粘度、减少加工时间并提高蛋白质产量的绝佳选择。例如，该酶与 BugBuster 和 PopCulture 蛋白质提取试剂兼容，因此可以与这些试剂一起添加，以消除大肠杆菌提取物中的粘度并去除核酸。

该酶包括两个 30kDa 的亚基。它在pH值6-10之间，温度0-42°C 起作用，并需要1-2mM Mg2+激活。该酶在离子和非离子型洗涤剂、还原剂、PMSF（1 mM）、EDTA（1 mM）和尿素（相对活性取决于特定条件）的存在下也具有活性。>150 mM单价阳离子，>100 mM磷酸盐，>100 mM硫酸铵或>100 mM盐酸胍能抑制其活性。

可提供超纯Benzonase 核酸酶（>99%，SDS-PAGE）和纯Benzonase核酸酶（>90%），标准浓度为25-29 U/µl，高浓度（HC）为250U/lµ。两种制剂均不含可检测的蛋白酶，并且比活度> 1 × 106蛋白。纯度>99%的产品经过内毒素测试，并含有<0.25 EU/1000 units. The product is suppliedas a 0.2 µm filtered solution in 50% glycerol.Store at -20°C.

Total endotoxin:<0.25 EU/1,000 units.Purity: >99%（SDS-PAGE）。

應用

用于去除蛋白样品中的核酸。

生化／生理作用

消化天然或热变性的DNA和RNA。

其他說明

一个单位定义为在 30 分钟内导致ΔA₂₆₀为1.0的酶量，这对应于37 µg DNA的完全消化。

法律資訊

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

毒性：标准处理（A）

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Rajeevkumar Raveendran Nair et al.
iScience, 23(3), 100888-100888 (2020-02-23)
Although a variety of remarkable molecular tools for studying neural circuits have recently been developed, the ability to deploy them in particular neuronal subtypes is limited by the fact that native promoters are almost never specific enough. We recently showed
William C Buchta et al.
Molecular neurobiology, 57(1), 346-357 (2019-07-31)
Cocaine addiction remains a major health concern with limited effective treatment options. A better understanding of mechanisms underlying relapse may help inform the development of new pharmacotherapies. Emerging evidence suggests that collapsin response mediator protein 2 (CRMP2) regulates presynaptic excitatory
Rodrigo Villaseñor et al.
Nature biotechnology, 38(6), 728-736 (2020-03-04)
Chromatin modifications regulate genome function by recruiting proteins to the genome. However, the protein composition at distinct chromatin modifications has yet to be fully characterized. In this study, we used natural protein domains as modular building blocks to develop engineered
Annabel Quinet et al.
Molecular cell, 77(3), 461-474 (2019-11-05)
Acute treatment with replication-stalling chemotherapeutics causes reversal of replication forks. BRCA proteins protect reversed forks from nucleolytic degradation, and their loss leads to chemosensitivity. Here, we show that fork degradation is no longer detectable in BRCA1-deficient cancer cells exposed to
Alexandros P Drainas et al.
Cell reports, 31(1), 107465-107465 (2020-04-09)
TP53 deficiency is the most common alteration in cancer; however, this alone is typically insufficient to drive tumorigenesis. To identify genes promoting tumorigenesis in combination with TP53 deficiency, we perform genome-wide CRISPR-Cas9 knockout screens coupled with proliferation and transformation assays

文章

Benzonase® 核酸酶問與答

本頁面列出 9 個關於 Benzonase® 核酸酶的常見問題與解答。

Benzonase® Nuclease Q&A

This page lists nine frequently asked questions and answers about Benzonase® Nuclease.

Use of BugBuster® and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria

The combination of BugBuster® and Lysonase™ reagents greatly enhances the release of soluble proteins from Gram-positive bacteria.

BugBuster® Plus Lysonase™ Kit: Cell Lysis and Nucleic Acid Removal for Gram-negative and Gram-positive Bacteria

Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit

查看全部

Questions

Reviews

No rating value

Active Filters

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務