After verification, an anti-doublecortin antibody recommended for free-floating application on mouse brain tissue is not currently available. However, a citation on AB2253 for free-floating application on mouse brain tissue was found and may be of interest for reference.
Product: AB2253 Sigma-Aldrich Anti-Doublecortin Antibody
Publication: Nat Commun. 2015 Mar 26;6:6606
Title: Aberrant hippocampal neurogenesis contributes to epilepsy and associated cognitive decline
PMID: 25808087
生物源
guinea pig
品質等級
共軛
unconjugated
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
rat, mouse
物種活性（以同源性預測）
human (based on 100% sequence homology)
技術
immunofluorescence: suitable (Frozen tissue sections)
immunohistochemistry: suitable (Paraffin)
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... DCDC1(341019)
1 of 4
本產品 | D9693 | MABN707 | ABN90 |
|---|---|---|---|
| biological source guinea pig | biological source rabbit | biological source mouse | biological source guinea pig |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| antibody form serum | antibody form affinity isolated antibody | antibody form purified antibody | antibody form serum |
| shipped in wet ice | shipped in dry ice | shipped in wet ice | shipped in wet ice |
| clone polyclonal | clone polyclonal | clone 2G5, monoclonal | clone polyclonal |
| species reactivity rat, mouse | species reactivity human, mouse, rat | species reactivity mouse, human | species reactivity rat, mouse |
一般說明
抗双皮质素抗体，目录号AB2253，是检测双皮质素的豚鼠多克隆抗体，经测试可用于免疫组化（石蜡）、免疫荧光和蛋白质印迹中。
目标分子量：观察值为~42 kDa；计算值为40.61 kDa。在一些裂解液中可能会观察到未表征的条带。
观察分子量为〜45 kDa；计算分子量为40.57 kDa。在某些裂解物中可以观察到未鉴定的条带。
免疫原
表位：C端
重组肽。
應用
抗双皮质素抗体已被用于免疫组化和免疫荧光染色中。
生化／生理作用
Doublecortin(DCX)是神经发生的重要标志物。它是研究和比较不同物种的神经发生过程的宝贵工具。[1]
与其他物种的反应性尚未确定。
这种豚鼠多克隆抗体可检测鼠和大鼠物种中的双皮质素。它靶向的是一个在C末端区域含有17个氨基酸的表位。
外觀
GST去除豚鼠血清，含0.05%叠氮化钠
GST耗尽
準備報告
自收到之日起在-20°C可稳定保存1年。使用建议：收到后，在取下瓶盖之前，将小瓶离心并轻轻混合溶液。分装到微量离心管中，并储存于 -20°C。避免反复冻融循环，以免损坏 IgG 和影响产品性能。
分析報告
对照
大鼠脑裂解液。
大鼠脑裂解液。
通过蛋白质印迹在大鼠脑组织裂解液中进行了评估。
蛋白印迹分析：该抗体的1：1,000稀释液可在大鼠脑组织裂解液中检测到双皮质素。
蛋白印迹分析：该抗体的1：1,000稀释液可在大鼠脑组织裂解液中检测到双皮质素。
其他說明
替代品：AB5910
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Embryonic Nkx2. 1-expressing neural precursor cells contribute to the regional heterogeneity of adult V-SVZ neural stem cells
Delgado RN and Lim DA
Developmental Biology, 407(2), 265-274 (2015)
Tomás P Bachor et al.
Neurobiology of disease, 108, 13-28 (2017-07-27)
Diabetes mellitus (DM) is reaching epidemic conditions worldwide and increases the risk for cognition impairment and dementia. Here, we postulated that progenitors in adult neurogenic niches might be particularly vulnerable. Therefore, we evaluated the different components of the mouse subventricular
Combination treatment with ethyl pyruvate and IGF-I exerts neuroprotective effects against brain injury in a rat model of neonatal hypoxic-ischemic encephalopathy
Rong Z, et al.
International Journal of Molecular Medicine, 36(1), 195-203 (2015)
Jacques Balthazart et al.
Brain, behavior and evolution, 84(1), 1-4 (2014-07-19)
Commentary on “Evaluating the Predictive Value of Doublecortin as a Marker for Adult Neurogenesis in Canaries (Serinus canaria)” by Michiel Vellema, Moritz Hertel, Susan L. Urbanus, Annemie Van der Linden, and Manfred Gahr (Journal of Comparative Neurology 2014, 522 :
Hanadie Yousef et al.
Oncotarget, 6(14), 11959-11978 (2015-05-25)
Stem cell function declines with age largely due to the biochemical imbalances in their tissue niches, and this work demonstrates that aging imposes an elevation in transforming growth factor β (TGF-β) signaling in the neurogenic niche of the hippocampus, analogous
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Is it possible to obtain a small aliquot of specific antibodies for testing purposes to determine their suitability for staining Doublecortin (DCX) in immunohistochemistry on free-floating mouse brain tissue fixed with PFA? The antibodies of interest are the guinea pig polyclonal and the goat polyclonal.
1 answer-
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