ABE553

Anti-CREB-1 Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human, rat
• 物種活性（以同源性預測）: porcine (based on 100% sequence homology), equine (based on 100% sequence homology)
• 技術: ChIP: suitable; immunocytochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_004370
• UniProt登錄號: P16220
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CREB1(1385)

描述

一般說明

Cyclic AMP-responsive element-binding protein (CREB) is a beta ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2.

~51 kDa observed. A doublet has been observed at 48 kDa in some cell lysates (Rutberg, S. E., et al. (1999). Oncogene. 18(8):1569-1579.). Uncharacterized bands may be observed in some cell lysates.

免疫原

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human CREB-1.

應用

Detect CREB using this rabbit polyclonal antibody, Anti-CREB-1 Antibody validated for use in western blotting & ICC.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected CREB-1 in PC-12 cells.

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB-1 in HEK293T cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

生化／生理作用

This antibody recognizes the N-terminus of CREB-1.

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

準備報告

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
A431 cell lysate

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CREB-1 in 10 µg of A431 cell lysate.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-218

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable