ABN2252

Anti-Neuroligin-4

• 同義詞: Neuroligin Y
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human
• 包裝: antibody small pack of 100
• 技術: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal cytoplasmic domain
• Protein ID登錄號: NP_055708
• UniProt登錄號: Q8NFZ3
• 目標翻譯後修改: unmodified
• 基因資訊: human ... NLGN4Y(22829)

描述

一般說明

Neuroligin-4 (UniProt: Q8NFZ3; also known as Neuroligin Y) is encoded by the NLGN4Y (also known as KIAA0951) gene (Gene ID: 22829) in human. Neuroligins are postsynaptic cell adhesion molecules that have crucial roles in synapse maturation and function. In humans, NLGN4X is located on the X chromosome and the NLGN4Y is localized on the Y chromosome and together they form an X-Y pair. They share about 97% amino acid identity; however, they are differentially regulated. Although several mutations in NLGN4X have been linked to autism, only one mutation has been identified in NLGN4Y gene. Despite their high degree of sequence identity, NLGN4Y exhibits severe deficit in trafficking to cell surface and related synaptic effects. This has been attributed to serine 93 in NLGN4Y. A substitution of serine to proline at residue 93 is shown to rescue it and increase its surface trafficking. Neuroligin-4 is a single-pass type I, homodimeric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-43), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 44-676), a transmembrane domain (aa 677-697), and a cytoplasmic domain (aa 698-816). Its expression is observed in fetal and adult brain, prostate, and testis. It serves as a neuronal cell surface protein that is involved in cell-cell interactions. (Ref.: Nguyen, TA., et al. (2020). Neuron 106(5); 759-768; Marro, SG., et al. (2019). Neuron. 103(4); 617-626).

免疫原

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal, cytoplasmic domain of human Neuroligin-4.

應用

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293T cells overexpressing Human Neuroligin-4.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Neuroligin-4 in lysate from HEK293T cells overexpressing Human Neuroligin-4.

Tested Applications

Immunofluorescence Analysis: A representative lot detected Neuroligin-4 in Immunofluorescence applications (Nguyen, T.A., et. al. (2020). Neuron. 106(5):759-768).

Western Blotting Analysis: A representative lot detected Neuroligin-4 in Western Blotting applications (Nguyen, T.A., et. al. (2020). Neuron. 106(5):759-768).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Neuroligin-4 in Immunoprecipitation applications (Nguyen, T.A., et. al. (2020). Neuron. 106(5):759-768).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

生化／生理作用

This rabbit polyclonal antibody detects Neuroligin-4. It targets an epitope within 16 amino acids from the C-terminal, cytoplasmic domain.

外觀

Purified rabbit polyclonal antibody in buffer containing PBS, pH 7.4, without azide.

準備報告

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable