APT129
Caspase 8 Colorimetric Activity Assay Kit 25, IETD
Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD.
同義詞:
FLICE
跳轉至
物種活性（以同源性預測）
mammals
製造商／商標名
Chemicon®
技術
activity assay: suitable
UniProt登錄號
檢測方法
colorimetric
運輸包裝
dry ice
一般說明
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon′s Caspase-8 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity.
應用
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
準備報告
Store kit materials at -20°C up to their expiration date.
Special Precautions:
· After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Avoid repeated freeze/thaw cycles.
· The Caspase-8 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
Special Precautions:
· After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Avoid repeated freeze/thaw cycles.
· The Caspase-8 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
其他說明
5X Cell Lysis Buffer (Part No. 90065): 5 mL
5X Assay Buffer (Part No. 90066): 10 mL
Caspase-8 Substrate (Ac-IETD-pNA) (Part No. 90081): 250 μL of 2.5 mg/mL solution
Caspase-8 Inhibitor (Ac-IETD-CHO) (Part No. 90086): 50 μL of 100 mM (0.05 mg/mL) in DMSO
pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
5X Assay Buffer (Part No. 90066): 10 mL
Caspase-8 Substrate (Ac-IETD-pNA) (Part No. 90081): 250 μL of 2.5 mg/mL solution
Caspase-8 Inhibitor (Ac-IETD-CHO) (Part No. 90086): 50 μL of 100 mM (0.05 mg/mL) in DMSO
pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
10 - Combustible liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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