FCMAB113A4

Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor^™ 488 conjugate

clone 10H11.2, from mouse, ALEXA FLUOR^™ 488

• 同義詞: Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 共軛: ALEXA FLUOR^™ 488
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 10H11.2, monoclonal
• 物種活性: human
• 技術: flow cytometry: suitable; immunocytochemistry: suitable
• 同型: IgG1
• NCBI登錄號: NM_002701.4
• UniProt登錄號: Q01860
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... POU5F1(5460)

描述

一般說明

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent embryonic stem (ES) and embryonic carcinoma (EC) cells express Oct-4. Additionally, murine embryonic germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.

~44 kDa

免疫原

Recombinant GST fusion protein, human Oct-4 (aa 1-134).

應用

Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate validated for use in FC & IC.

Flow Cytometry:
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments

Working Solution	Cells / test	Total Reaction Volume
5 μl	5 x 105 Cells / 95 μl PBS	100 μl
10 μl	1 x 106 Cells / 90 μl PBS	100 μl

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

生化／生理作用

Reacts with human Oct-4.

外觀

Purified mouse monoclonal IgG1 conjugated to Alexa Flour 488 in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.

準備報告

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt

分析報告

Control
Human embryonic stem cells

Evaluated by Flow Cytometry in 2102Ep Cells.

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable