MAB353B

Anti-Nestin Antibody, clone rat-401, Biotin Conjugate

clone rat-401, from mouse, biotin conjugate

• 同義詞: rat401, NES, Nbla00170, FLJ21841
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 共軛: biotin conjugate
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: rat-401, monoclonal
• 物種活性: mouse, rat
• 應無反應活性: human
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable
• 同型: IgG1
• NCBI登錄號: NP_037119
• UniProt登錄號: P21263
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... NES(10763) ; mouse ... Nes(18008) ; rat ... Nes(25491)

描述

一般說明

200-220 kDa calculated

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.

免疫原

Embryonic rat spinal cord

應用

Detect Nestin using this mouse monoclonal antibody, Anti-Nestin Antibody, clone rat-401, Biotin Conjugate validated for use in ICC & IHC.

Evaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022).
Immunocytochemsitry Analysis: A 1:200 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).
Evaluated by Immunocytochemistry in ReNcell VM human neural progenitor cells (SCC008).
Immunocytochemsitry Analysis: A 1:200 dilution of this antibody did not detect Nestin in ReNcell VM human neural progenitor cells (SCC008). Evaluated by Immunohistochemistry in rat hindbrain brain tissue.
Immunohistochemsitry Analysis: A 1:50 dilution of this antibody detected Nestin in rat hindbrain brain tissue.

Research Category
Stem Cell Research

Neuroscience

Research Sub Category
Developmental Neuroscience

Neural Stem Cells

生化／生理作用

This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.

外觀

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15mg/ml BSA.

準備報告

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

分析報告

Control
Mouse cortical neural stem cells (SCR029)

Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:200 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable