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Merck

MAB5270

Anti-Choline Acetyltransferase Antibody

CHEMICON®, mouse monoclonal, 1.B3.9B3

同義詞:

ChAT

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分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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產品名稱

Anti-Choline Acetyltransferase Antibody, clone 1.B3.9B3, clone 1.B3.9B3, Chemicon®, from mouse

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

1.B3.9B3, monoclonal

物種活性

human, rat, pig

製造商/商標名

Chemicon®

技術

ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

同型

IgG1

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... CHAT(1103)
pig ... Chat(396896)
rat ... Chat(290567)

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1 of 4

本產品
AB1582MAB5350AB15468
biological source

mouse

biological source

sheep

biological source

mouse

biological source

chicken

Gene Information

human ... CHAT(1103)
pig ... Chat(396896)
rat ... Chat(290567)

Gene Information

rabbit ... Chat(100342226)
rat ... Chat(290567)

Gene Information

human ... CHAT(1103)
rat ... Chat(290567)
rhesus monkey ... Chat(709977)

Gene Information

mouse ... Chat(12647)

clone

1.B3.9B3, monoclonal

clone

polyclonal

clone

28C4, monoclonal

clone

polyclonal

species reactivity

human, rat, pig

species reactivity

rat, guinea pig, rabbit

species reactivity

human, rat, guinea pig, monkey

species reactivity

mouse

antibody form

purified immunoglobulin

antibody form

serum

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

一般說明

69 kDa

免疫原

Highly purified ChAT obtained from porcine brain (Ostermann-Fatif et al., 1990).

應用

Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-Choline Acetyltransferase Antibody, clone 1.B3.9B3 is validated for use in ELISA, IH, IH(P), WB for the detection of Choline Acetyltransferase.
Western blot

ELISA

Immunohistochemistry: 10-20 μg/mL in paraffin embedded sections {See Ratcliffe et al. 1998}

Optimal working dilutions and protocols must be determined by end user.

生化/生理作用

Reacts with choline acetyltransferase (ChAT)-positive cells from humans, rats and pigs (Ostermann-Fatif et al., 1992a,b).

外觀

Protein A purified
Format: Purified
Purified immunoglobulin. Lyophilized from 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide. Reconstitute to 100 μg/mL with sterile distilled water.

準備報告

Maintain lyophilized antibody at 2–8°C in undiluted aliquots for up to 6 months. Maintain reconstituted antibody at -20°C in undiluted aliquots for up to 6 months.

分析報告

Control
Brain tissue

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Xue-Lian Qi et al.
Cell reports, 36(5), 109469-109469 (2021-08-05)
Acetylcholine plays a critical role in the neocortex. Cholinergic agonists and acetylcholinesterase inhibitors can enhance cognitive functioning, as does intermittent electrical stimulation of the cortical source of acetylcholine, the nucleus basalis (NB) of Meynert. Here we show in two male
Lydia Lebenheim et al.
Molecular psychiatry (2020-11-16)
The striatum is the main input structure of the basal ganglia. Distinct striatal subfields are involved in voluntary movement generation and cognitive and emotional tasks, but little is known about the morphological and molecular differences of striatal subregions. The ventrolateral
Angela Marina Montalbano et al.
Mediators of inflammation, 2016, 9063842-9063842 (2016-06-15)
IL-17A is overexpressed in the lung during acute neutrophilic inflammation. Acetylcholine (ACh) increases IL-8 and Muc5AC production in airway epithelial cells. We aimed to characterize the involvement of nonneuronal components of cholinergic system on IL-8 and Muc5AC production in bronchial
Julio Santos-Torres et al.
Journal of neurotrauma, 26(9), 1593-1607 (2009-06-16)
Lesions in specific areas of the rat motor cortex generate deficits related to fine movement performance affecting the forelimb. We have previously shown that transplants of embryonic frontal cortex ameliorate these motor deficits. Amelioration has been associated with a functional
Katarina Zvarova et al.
The Journal of comparative neurology, 489(4), 501-517 (2005-07-19)
We examined the distribution and fate of cocaine- and amphetamine-regulated transcript peptide (CARTp)(55-102)-immunoreactive (IR) structures in the neonatal and adult rat urinary bladder. Double-labeling studies examining CARTp with tyrosine hydroxylase (TH), neuronal nitric oxide synthase (nNOS), or choline acetyltransferase (ChAT)

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