QIA127

Rapid Cell Proliferation Kit

• 同義詞: Proliferation assay kit
• 分類程式碼代碼: 12352207
• NACRES: NA.84

屬性

• 用途: sufficient for 500 tests
• 品質等級: 100
• 包裝: pkg of 1 96-well plate(s)
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• 輸入: sample type cultured cells (1,000-50,000 cells)
• 檢測方法: colorimetric
• 運輸包裝: wet ice
• 儲存溫度: −20°C

描述

一般說明

Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.

生化／生理作用

The Calbiochem Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.

特點和優勢

Assay Time: 0.5 - 4 h

Sensitivity: 1000 cells (adherent); 2500 cells (nonadherent)

準備報告

• WST-1 Labeling Mixture: Thaw the EMS solution at room temperature or in a 37°C water bath. Add the entire contents of this solution to the bottle containing the WST-1 reagent. The mixed solution can be stored at 4°C for several weeks. For long-term storage (several months) aliquot and store at -20°C. Protect from light.

其他說明

Ishiyama, M., et al. 1995. In Vitro Toxicology8, 187.
Liu, S.O., et al 1995. Nat. Med.1, 267.
Ishiyama, M., et al. 1993. Chem. Pharm. Bull.41, 1118.

WST-1, 1-Methoxy PMS, and a user protocol.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

安全資訊

• 儲存類別代碼: 10 - Combustible liquids