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Merck

SLM-241

DMEM - high glucose

Complete medium, with ʟ-glutamine and sodium pyruvate and 10% FBS, liquid, 0.2 μm filtered, suitable for cell culture

同義詞:

DME, DMEM with 10% FBS, Dulbecco′s Modified Eagle′s Medium - high glucose

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分類程式碼代碼:
12352207
eCl@ss:
32160801
NACRES:
NA.75

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產品名稱

DMEM Complete Medium, with 2mM L-Glut, Sodium Pyruvate, and 10% FBS, contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS

品質等級

300

形狀

liquid

技術

cell culture | mammalian: suitable

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本產品
SLM-244D0822SLM-245
DMEM Complete Medium, with 2mM L-Glut, Sodium Pyruvate, and 10% FBS contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS

Sigma-Aldrich

SLM-241

DMEM Complete Medium, with 2mM L-Glut, Sodium Pyruvate, and 10% FBS

MEM Complete Medium, with NEAA, 1 mM sodium pyruvate, 2mM L-Glut, and 10% FBS contains non-essential amino acids, 1mM sodium pyruvate, 2mM L-glut, and 10% U.S. origin FBS

Sigma-Aldrich

SLM-244

MEM Complete Medium, with NEAA, 1 mM sodium pyruvate, 2mM L-Glut, and 10% FBS

Sigma-Aldrich

Sigma-Aldrich

D0822

StableCell™ DMEM - 高葡萄糖

MEM Complete Medium, with NEAA, 2mM L-Glut and 10% FBS contains non-essential amino acids, 2mM L-glutamine and 10% U.S. origin FBS

Sigma-Aldrich

SLM-245

MEM Complete Medium, with NEAA, 2mM L-Glut and 10% FBS

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

300

Quality Level

300

Quality Level

400

Quality Level

300

一般說明

Dulbecco′s Modified Eagle (DMEM) Complete Medium contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS. DMEM Complete Medium is a convenient 0.2 μm filtered solution for the culture of primary mammalian cells including mouse and chicken cells, and a variety of normal and transformed cells.

應用

DMEM Complete Medium, with 2mM L-Glut, Sodium Pyruvate, and 10% FBS has been used:
  • to culture human gingival fibroblasts[1]
  • to culture mouse fibroblast cells[2]
  • as an overlay medium for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plaque assay[3]

分析報告

pH= 7.2-7.5
Osmolality= 320-346
Endotoxin= ≤ 2EU/mL
Mycoplasma= Negative
Sterility Tested= Negatvie for Growth
Appearance= Orange/ Red Clear Liquid
Cell Line Tested= HT-22 Human Neuronal Cells

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Sumaira Javaid et al.
PloS one, 14(11), e0225056-e0225056 (2019-11-20)
Natural flora is the richest source of novel therapeutic agents due to their immense chemical diversity and novel biological properties. In this regard, eighteen natural products belonging to different chemical classes were evaluated for their thymidine phosphorylase (TP) inhibitory activity.
Cytotoxicity and Collagen Expression Effects of Tideglusib Administration on Human Periodontal Cells: An In-Vitro Study
Buse ONCU, et al.
Clinical and Experimental Medical Sciences, 10(2), 153-162 (2020)
Juan A Perez-Bermejo et al.
Science translational medicine, 13(590) (2021-03-17)
Although coronavirus disease 2019 (COVID-19) causes cardiac dysfunction in up to 25% of patients, its pathogenesis remains unclear. Exposure of human induced pluripotent stem cell (iPSC)-derived heart cells to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) revealed productive infection and
Leonard F Blackwell et al.
Frontiers in public health, 6, 153-153 (2018-06-16)
The patterns of a woman's normal ovarian activity can take many forms from childhood to menopause. These patterns lie on a continuum ranging from no ovarian activity to a fully fertile ovulatory cycle, but among the other defined patterns are

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CellASIC® ONIX2 微流控系統如何用於分析 caspase-3 介導的細胞凋亡/細胞死亡及活體免疫和癌症細胞系的細胞缺氧的應用說明。

Dynamic Live Cell Imaging of Caspase Mediated Apoptosis and Cellular Hypoxia in Lymphocytes and Cancer Cells Using the CellASIC® ONIX2 Microfluidic Platform

Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.

Questions

1–3 of 3 Questions  

  1. What is the composition of this product?

    1 answer

    1. The product is a comprehensive DMEM formulation that includes high glucose with 2mM L-glutamine, sodium pyruvate, and 10% FBS.

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  2. Can you provide the formulation for this media?

    1 answer

    1. The formulation of this media is proprietary. The following is known:

      Glucose: 4.5g/L (high Glucose Formulation)

      If you need to determine the presence or absence of a particular component or its concentration, please contact Technical Service with justification. For example, if the knowledge will support a specific downstream assay, we will then request this information. Please note that we cannot guarantee that this will be provided.

      Helpful?

  3. What is the formulation information available as compared to other DMEMs?

    1 answer

    1. This product is considered a complete DMEM formulation containing high glucose with 2mM L-glutamine, sodium pyruvate, and 10% FBS. Please see the link below to review and compare other classical DMEM formulations: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/380/910/d0572for.pdf

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