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Merck

UFC201024

Amicon® Ultra离心超滤管，10 kDa MWCO

greener alternative

sample volume 2 mL, regenerated cellulose membrane, MWCO 10 kDa

同義詞:

离心浓缩器，10 kDa MWCO，2 mL样品体积

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分類程式碼代碼:
41104916
eCl@ss:
36100101
NACRES:
NB.24

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材料

polypropylene tube (for collection)
regenerated cellulose membrane
styrene-butadiene housing

品質等級

400

無菌

non-sterile

產品線

Amicon®

包裝

pkg of 24 pkg

製造商／商標名

Amicon® Ultra

sustainability

Greener Alternative Product

參數

2 mL sample volume

技術

protein extraction: suitable
protein purification: suitable

長度

119.7 mm

直徑

15.9 mm

過濾面積

1 cm2

容積

2 mL

有效容積

≤2 mL
2 mL

孔徑

10 kDa MWCO

環保替代類別

Re-engineered

運輸包裝

ambient

相關類別

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Show Differences

1 of 4

本產品
UFC8010UFC5010UFC9010
Amicon® Ultra离心超滤管，10 kDa MWCO sample volume 2 mL, regenerated cellulose membrane, MWCO 10 kDa

Millipore

UFC201024

Amicon® Ultra离心超滤管，10 kDa MWCO

Amicon® Ultra过滤器，10 kDa MWCO sample volume 4 mL, regenerated cellulose membrane, MWCO 10 kDa

Millipore

UFC8010

Amicon® Ultra过滤器，10 kDa MWCO

Amicon® Ultra过滤器，10 kDa MWCO sample volume 0.5 mL, regenerated cellulose membrane, MWCO 10 kDa

Millipore

UFC5010

Amicon® Ultra过滤器，10 kDa MWCO

Amicon® Ultra过滤器，10 kDa MWCO sample volume 15 mL, regenerated cellulose membrane, MWCO 10 kDa

Millipore

UFC9010

Amicon® Ultra过滤器，10 kDa MWCO

pore size

10 kDa MWCO

pore size

10 kDa MWCO

pore size

10 kDa MWCO

pore size

10 kDa MWCO

parameter

2 mL sample volume

parameter

4 mL sample volume

parameter

0.5 mL sample volume

parameter

15 mL sample volume

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

material

polypropylene tube (for collection), styrene-butadiene housing, regenerated cellulose membrane

material

polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing

material

polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing

material

polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing

product line

Amicon®

product line

Amicon®

product line

Amicon®

product line

Amicon®

一般說明

Amicon® Ultra-2离心过滤装置提供快速超滤。它具有从稀释的和复杂的样品基质中回收高浓度因子和轻松浓缩的潜力。Amicon® Ultra-2设备未经消毒，仅供一次使用。
我们竭诚为您带来经过重新设计的绿色替代产品，实现可持续性更高的研究方案。 内部过滤装置采用按ISCC质量平衡分配的生物循环材料制成。点击此处以获取更多信息。

應用

  • 采用10,000 Dalton分子量截止超滤®膜进行超滤。
  • 含抗原、抗体、酶、核酸或微生物的生物样品的浓度。
  • 纯化在组织培养提取物或细胞裂解液中发现的大分子成分。
  • 引物去除、PCR清理、从反应混合物中去除连接物或大分子标记，以及在HPLC之前去除蛋白质。
  • 脱盐，缓冲液更换和蛋白质透析。
  • 体积排阻和蛋白质分离。
Amicon® Ultra-2离心过滤装置已用于：
  • 在细胞外囊泡(EV)分离前减少大量生物液体或浓缩细胞外囊泡[1]
  • 浓缩含抗原、抗体、酶、核酸(DNA/RNA样品，单链或双链)、微生物、柱洗脱物和纯化样品的生物样品
  • 在高效液相色谱(HPLC)分析之前，对组织培养提取物和细胞裂解液中发现的大分子成分进行纯化，从反应混合物中去除引物、连接物或分子标记，去除蛋白质
  • 脱盐、缓冲交换[2]或过滤

特點和優勢

  • Amicon® Ultra 2 ml 10K 24支包装
  • 可浓缩2 mL至15-70 μl
  • 98%的样品回收率
  • 快速的处理时间-10至30分钟的离心时间
  • 工程化永停点
  • 反向离心功能可提供一致的回收率

  • 使用10 kda NMWCO的Ultracel®再生纤维素膜提供大于90%的保留回收率
  • 2 mL浓缩至15-70 μL
  • 98％的样品回收率
  • 快速处理时间：10至30分钟旋转时间
  • 工程化永停点
  • 反向旋转能力提供一致的回收率

其他說明

Amicon® Ultra-2装置随附两根管。在操作过程中，一根管用来回收滤液；另一根管在浓缩过程中用来封盖设备，随后回收浓缩样本。Amicon Ultra-2离心过滤装置仅供研究使用，不能用于诊断程序。
替代品：4205

法律資訊

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

分析證明 (COA)

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Bao Quoc Tran et al.
Data in brief, 6, 68-76 (2016-01-23)
A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated
Keerthie Dissanayake et al.
Theriogenology, 149, 104-116 (2020-04-08)
Extracellular vesicles (EVs) are membrane-bound biological nanoparticles (NPs) and have gained wide attention as potential biomarkers. We aimed to isolate and characterize EVs from media conditioned by individually cultured preimplantation bovine embryos and to assess their relationship with embryo quality.
Lulai Xu et al.
Vaccine, 38(7), 1690-1699 (2020-01-16)
Newcastle disease virus (NDV) has been used as a vector in the development of vaccines and gene delivery. In the present study, we generated the thermostable recombinant NDV (rNDV) expressing the different forms of hemagglutinin (HA) of highly pathogenic avian
Glenn Vergauwen et al.
Scientific reports, 7(1), 2704-2704 (2017-06-04)
Identification and validation of extracellular vesicle (EV)-associated biomarkers requires robust isolation and characterization protocols. We assessed the impact of some commonly implemented pre-analytical, analytical and post-analytical variables in EV research. Centrifugal filters with different membrane types and pore sizes are
Jasjeet Kaur et al.
Virus genes, 56(4), 480-497 (2020-05-06)
Staphylococcus aureus is one of the most dreadful infectious agents, responsible for high mortality and morbidity in both humans and animals. The increased prevalence of multidrug-resistant (MDR) Staphylococcus aureus strains has limited the number of available treatment options, which calls

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Questions

1–3 of 3 Questions  

  1. At what speed should the Amicon® Ultra Centrifugal Filter, 10 kDa MWCO UFC201024 be used? Besides, what is the maximun g-force supported by the Amicon?

    1 answer

    1. The speed needs to be evaluated. However, we know that the maximum speed should never be exceeded. It is best to run below the maximum speed to not risk breakage in case the centrifuge has not been calibrated recently. Here are our specifications for the Amicon Ultra 0.2: Maximum relative centrifugal force Swinging bucket rotor 4,000 × g for concentration spin, 1,000 × g for recovery spin Fixed angle rotor 7,500 × g for concentration spin, 1,000 × g for recovery spin
      NOTE: When spinning viscous solutions such as undiluted serum or plasma, do not exceed 5,400 x g. Furthermore, if you are trying to concentrate proteins, often, proteins do not like to be spun at a high speed or they can precipitate. Therefore, let's say your centrifuge is run at 2000 x g. This should work fine. It may take longer or it may not. This depends on initial and final desired volume when stopped.  

      Helpful?

  2. Can Amicon® Ultra Centrifugal Filter, 10 kDa MWCO UFC201024 to be in the Centrifuge as 4 mL Centrifugal Filter without adaptors？We have adaptors for 15 mL TUBES

    1 answer

    1. Amicon Ultra-2 devices should be used in a centrifuge with swinging bucket or fixed angle rotor with wells/carriers that can accommodate 17 mm × 100 mm tubes. This is the same well/carrier size that is recommended for the Amicon® Ultra-4 devices. To avoid damage to the device during centrifugation, make sure it is properly assembled and seated at the bottom of the rotor. The rim of the concentrate collection tube should be inside the rotor well. Check clearance before spinning.

      Helpful?

  3. Hello. Does these 2 ml 10 kDa filters fit into the same centrifuge rotor as the 0.5 ml 10 kDa amicon filters?

    1 answer

    1. No, the Amicon-0.5 devices require a microfuge, while the Amicon-2 devices require a centrifuge with adapters for 5 ml tubes.

      Helpful?

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