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Merck

UFC9030

Amicon® Ultra离心超滤管，30 kDa MWCO

greener alternative

sample volume 15 mL, regenerated cellulose membrane, MWCO 30 kDa

Amicon® Ultra Centrifugal Filter, 30 kDa MWCO

同義詞:

15 mL样品体积, 30 kDa MWCO, 离心浓缩器

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分類程式碼代碼:
41104916
eCl@ss:
36100101
NACRES:
NB.24

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材料

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

品質等級

400

無菌

non-sterile

產品線

Amicon®

包裝

pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg

製造商／商標名

Amicon® Ultra

sustainability

Greener Alternative Product

參數

15 mL sample volume

技術

protein extraction: suitable
protein purification: suitable

長度

12.1 cm (4.807 in.)

直徑

2.97 cm

過濾面積

7.6 cm2

容積

15 mL

有效容積

≤15 mL
15 mL

孔徑

30 kDa MWCO

環保替代類別

Re-engineered

運輸包裝

ambient

相關類別

比較類似項目

查看完整比較

Show Differences

1 of 4

本產品
UFC8030UFC203024UFC5030
Amicon® Ultra离心超滤管，30 kDa MWCO sample volume 15 mL, regenerated cellulose membrane, MWCO 30 kDa

Millipore

UFC9030

Amicon® Ultra离心超滤管，30 kDa MWCO

Amicon® Ultra离心超滤管，30 kDa MWCO sample volume 4 mL, regenerated cellulose membrane, MWCO 30 kDa

Millipore

UFC8030

Amicon® Ultra离心超滤管，30 kDa MWCO

Amicon® Ultra过滤器，30 kDa MWCO sample volume 2 mL, regenerated cellulose membrane, MWCO 30 kDa

Millipore

UFC203024

Amicon® Ultra过滤器，30 kDa MWCO

Amicon® Ultra过滤器，30 kDa MWCO sample volume 0.5 mL, regenerated cellulose membrane, MWCO 30 kDa

Millipore

UFC5030

Amicon® Ultra过滤器，30 kDa MWCO

parameter

15 mL sample volume

parameter

4 mL sample volume

parameter

2 mL sample volume

parameter

0.5 mL sample volume

material

polyethylene cap, polypropylene tube, regenerated cellulose support, polyethylene liner, styrene-butadiene housing, regenerated cellulose membrane

material

polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing

material

polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing

material

polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

pore size

30 kDa MWCO

pore size

30 kDa MWCO

pore size

30 kDa MWCO

pore size

30 kDa MWCO

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

product line

Amicon®

product line

Amicon®

product line

Amicon®

product line

Amicon®

一般說明

Amicon® Ultra 15 mL离心装置与Ultracel® 30000 Dalton分子量截止滤波器提供快速超滤，以及可靠的大分子保留。例如，1 mg/ml初始浓度下的卵白蛋白(45 kDa)保留率为>95%。

垂直设计和有效的膜表面积提供了快速的样品处理，高样品回收率（通常大于90%的稀起始溶液），以及80倍浓度的处理能力。垂直设计最大限度地减少了溶质极化和随后的膜污染，过滤装置中的物理永停点可防止纺丝干燥和潜在的样品损失。

使用移液器从过滤装置的样品库中收集浓缩液，同时将超滤液收集在所提供的离心管中。该装置可在摆动斗中旋转，也可在定角转子中旋转。Amicon® Ultra 15 mL过滤器采用垂直膜设计和永停点，可在短旋转时间内提供最高回收率。
我们竭诚为您带来经过重新设计的绿色替代产品，实现可持续性更高的研究方案。 内部过滤装置采用按ISCC质量平衡分配的60%生物循环材料。点击此处以获取更多信息。

應用

  • 采用30,000 Dalton分子量截止超滤®膜进行超滤。
  • 含抗原、抗体、酶、核酸或微生物的生物样品的浓度。
  • 纯化在组织培养提取物或细胞裂解液中发现的大分子成分，并在高效液相色谱法之前去除蛋白质。
  • 从柱洗脱液稀释或预先纯化的蛋白质的浓度。
  • 脱盐，缓冲液更换和蛋白质透析。

特點和優勢

  • 使用30 kda NMWCO的Ultracel®再生纤维素膜提供大于90%的保留回收率。
  • 垂直膜减少了超快旋转时间（快至10 - 15分钟）的浓度极化。
  • 热封膜减少了下游的可提取物。
  • 100%完整性测试，确保可靠性能。
  • 半透明外壳和体积刻度便于样品监测。
  • 直接移液器进样，省去了回收浓缩液的处理步骤。
  • 80–100X高浓缩系数。

外觀

色码：透明，带有粉色盖

法律資訊

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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您可以在文件庫中找到最近購買的產品相關文件。

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文章

蛋白質樣品超濾

探索超濾膜在蛋白質樣品製備方面的原理和優點，包括溶劑交換和樣品濃縮。

Protein Sample Ultrafiltration

Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.

奈米超濾

奈米粒子純化與生產中的過濾方法與膜過濾器選擇。

Nanoparticle Ultrafiltration

Filtration methods and membrane filter selection in nanoparticle purification and production.

條款

使用 Amicon® Ultra 離心過濾器快速濃縮抗體

免疫球蛋白的純化程序一般包括與蛋白質 A 或 G 色層析媒介親和、洗淨未結合的蛋白質，並使用低 pH 值緩衝液洗脫。

Rapid Antibody Concentration with Amicon® Ultra Centrifugal Filters

Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.

相關內容

樣品純化

用於下游分析的純化方法可降低樣品的複雜性、去除干擾、濃縮分析物質，以獲得更精確的結果。

樣品純化

用於下游分析的純化方法可降低樣品的複雜性、去除干擾、濃縮分析物質，以獲得更精確的結果。

Amicon® 超離心過濾器

濃縮蛋白質，避免流失。使用 Amicon® Ultra 過濾器進行濃縮時，能可靠地回收蛋白質。

Sample Purification

Purification methods for downstream analysis reduce sample complexity, remove interferences, and concentrate analytes for more accurate results.

2 Reviews This action will navigate to reviews.

Questions

1–8 of 8 Questions  

  1. how many units are there in 1 pkg

    1 answer

    1. This product is packaged as a box of 8 tubes, 24 tubes, or 96 tubes.

      Helpful?

  2. How many individual columns come in one package. For example, if I wanted to purchase the 24package option, wil I get 24 columns or 24 packages of x number of columns?

    1 answer

    1. A package of 24 would equate to 24 Ultra Centrifugal Filters.

      Helpful?

  3. Hi there, I need to extract exosome in the range of 30 to 150 nm. Which Ultra Centrifugal Filter is fit for my work? Thank you for your advice in advance.

    1 answer

    1. We have performed studies of nanometer particles and all of our studies show that using a 100 kda molecular weight cutoff should be fine to trap particles greater than 10 nm.  

      Helpful?

  4. Can this item be sterilised, and if so, what is the best method (e.g., autoclaving) to ensure that product function and integrity are maintained?

    1 answer

    1. This item cannot be autoclaved. Sterilization with Ethylene Oxide is possible, but the method has not been validated.

      Helpful?

  5. Can the product be washed and re-used?

    1 answer

    1. This product is intended for single use. Repeated usage may result in declining performance.

      Helpful?

  6. How many times can sample be added to the same filter unit? For example: If I have 100 mL cell free supernatant and want to add 12 mL at a time, centrifuge and collect retentate - followed by discarding the flow through and pouring the next 12 mL etc.

    1 answer

    1. The same Amicon filter unit can be used for repeated spins with the same sample. However, repeated spins may lead to increased clogging, resulting in slower flow rates and longer spin times.

      Helpful?

  7. I noticed no volume change after centrifuging 10mL cell media at 14,000xg for 20minutes. Could this be due to unsuccessful concentration?

    1 answer

    1. The lack of volume change suggests that the sample may contain a high amount of particulate matter, leading to rapid clogging and preventing effective concentration.

      Helpful?

  8. Hello! Our lab had to move to a different area of our building and we found some probably quite old boxes of Amicon Ultra 15s (Cat UFC903096, lot R8CN67048). Does this product have an expiration date/shelf life? If so, has this lot surpassed it?

    1 answer

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

2 Reviews This action will navigate to reviews.
1 out of 1 (100%) reviewers recommend this product

Reviews

Active Filters

  1. San Diego
    • Review 1
    • Votes 10
    5 out of 5 stars.

    Amicon Ultra-15-An excellent choice

    This was back ordered a long time, but it is an excellent concentrator.

    Helpful?

  2. 1 Ratings-only review

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