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Merck

PVP40

聚乙烯吡咯烷酮

average mol wt 40,000

同義詞:

PVP, 聚维酮

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線性公式:
(C6H9NO)n
CAS 編號:
MDL號碼:
分類程式碼代碼:
12161700
PubChem物質ID:
NACRES:
NA.25

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生物源

synthetic (oragnic)

品質等級

形狀

powder

分子量

average mol wt 40,000

雜質

<5.0% water (Karl Fischer)

黏度號碼

29-32(lit.)

溶解度

water: 100 mg/mL, clear, colorless to light yellow

應用

agriculture

SMILES 字串

C=CN1CCCC1=O

InChI

1S/C6H9NO/c1-2-7-5-3-4-6(7)8/h2H,1,3-5H2

InChI 密鑰

WHNWPMSKXPGLAX-UHFFFAOYSA-N

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1 of 4

本產品
PVP10RDD033437190
form

powder

form

powder

form

powder

form

powder

application(s)

agriculture

application(s)

-

application(s)

-

application(s)

-

biological source

synthetic (oragnic)

biological source

-

biological source

-

biological source

-

solubility

water: 100 mg/mL, clear, colorless to light yellow

solubility

-

solubility

-

solubility

-

mol wt

average mol wt 40,000

mol wt

average mol wt 10,000

mol wt

average mol wt 40,000

mol wt

average Mw ~1,300,000 by LS

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

一般說明

聚乙烯吡咯烷酮(PVP)可与酚类和生物碱形成复合物。该复合物的形成可实现从植物样品中进行酚类和生物碱的去除,从而抑制它们对蛋白的任何修饰以及它们可能在分光光度法对蛋白含量测定中引起的任何干扰。PVP还可能增强酶的稳定性。[1]
聚乙烯吡咯烷酮(PVP)是一种多功能合成聚合物,用于生命科学和生物化学研究。这种水溶性聚合物功能多样,可作为稳定剂、乳化剂、悬浮剂、增溶剂、保护性胶体和络合剂。其保护胶体的特性对于稳定蛋白、酶和其它生物物质有特别重要的价值。

應用

聚乙烯吡咯烷酮已用于从植物样品中纯化谷氨酰胺合成酶。[2]它已用于封闭PVDF(聚偏二氟乙烯)膜以避免非特异性结合。[3][4]

特點和優勢

  • 用于生命科学和生物化学研究的多功能表面活性剂
  • 优良的增溶剂和络合剂

其他說明

如需了解生化试剂系列的更多信息,请填写此表
聚乙烯吡咯烷酮是 Denhardt′s 溶液的一种成分,在标准 50X 储备溶液中的浓度为 1%(w/v)。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

Lot/Batch Number

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Structural analysis of N- and O-glycans released from glycoproteins.
Jensen PH et al.
Nature Protocols, 7, 1299-1299 (2012)
Manna Crespan et al.
Scientific reports, 10(1), 7206-7206 (2020-05-01)
'Glera' and 'Ribolla Gialla' are the most economically relevant local grapevine cultivars of Friuli Venezia Giulia region (north-eastern Italy). 'Glera' is used to produce the world-renowned Prosecco wine. 'Ribolla Gialla' cultivation is constantly increasing due to the strong demand for
Angiotensin II inhibits insulin signaling in aortic smooth muscle cells at multiple levels. A potential role for serine phosphorylation in insulin/angiotensin II crosstalk.
Folli F et al.
The Journal of Clinical Investigation, 100, 2158-2158 (1997)
Quantification of the Antioxidant Activity in Salt-Stressed Tissues.
Maksimovic JJD and Zivanovic BD
Plant Salt Tolerance: Methods and Protocols, 237-237 (2012)
Glutamine Synthetases of Higher Plants : Evidence for a Specific Isoform Content Related to Their Possible Physiological Role and Their Compartmentation within the Leaf.
McNally SF et al.
Plant Physiology, 72, 22-22 (1983)

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學習 Northern 和 Southern 印迹的基本知識,以及將大分子轉移到膜支持物上的協議和應用。

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Questions

1–4 of 4 Questions  
  1. What is the stability for this product after reconstitution in water or medium? Is it possible to keep it at +4°C or aliquot at -20°C? If so, how long?

    1 answer
    1. Solutions of this product in water can be stored at room temperature for up to a year. The solution could also be stored in the refrigerator at 2-8°C, if so desired. But the recommendation would be to NOT freeze the solution, as this might shear the polymer, which would give a lower average molecular weight, and therefore lower viscosity.

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  3. I'm interested in using this product for reducing the inhibitory effects of polyphenols during ELISA extraction of walnuts. Do you think this product would help this?

    1 answer
    1. This product has not been tested for reducing inhibitory effects of polyphenols during ELISA extraction of walnuts. However, literature suggests that polyvinylpyrrolidone (PVP) may be effective, although the molecular weight used is not specified. The reference is: Abiodun A. Ogunjimi, Prabhakara V. Choudary, "Adsorption of endogenous polyphenols relieves the inhibition by fruit juices and fresh produce of immuno-PCR detection of Escherichia coli 0157:H7," FEMS Immunology & Medical Microbiology, Volume 23, Issue 3, March 1999, Pages 213–220.

      Contacting the article's author may provide information on the specific product number used in the experiment.

      Helpful?

  4. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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