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Merck

646547

三(2-羧乙基)膦 盐酸盐 溶液

0.5 M, pH 7.0(aqueous solution; pH was adjusted with ammonium hydroxide)

同義詞:

TCEP

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經驗公式(希爾表示法):
C9H15O6P · HCl
CAS 編號:
分子量::
286.65
MDL號碼:
分類程式碼代碼:
12352128
PubChem物質ID:
NACRES:
NA.25

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濃度

0.5 M

折射率

n20/D 1.367

pH值

7.0(aqueous solution; pH was adjusted with ammonium hydroxide)

密度

1.041 g/mL at 25 °C

SMILES 字串

Cl.OC(=O)CCP(CCC(O)=O)CCC(O)=O

InChI

1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H

InChI 密鑰

PBVAJRFEEOIAGW-UHFFFAOYSA-N

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1 of 4

本產品
68957C4706580560
Quality Level

200

Quality Level

100

Quality Level

300

Quality Level

100

density

1.041 g/mL at 25 °C

density

-

density

1.041 g/cm3 at 25 °C

density

-

refractive index

n20/D 1.367

refractive index

-

refractive index

-

refractive index

-

concentration

0.5 M

concentration

-

concentration

-

concentration

-

pH

7.0(aqueous solution; pH was adjusted with ammonium hydroxide)

pH

-

pH

-

pH

-

一般說明

三(2-羧乙基)膦(TCEP)能够在水溶液中高效裂解二硫键。[1]与其他三烷基膦(三丁基膦)不同,TCEP溶于水且无味。它的毒性也比2-巯基乙醇更低。这些优点使其成为更好的还原剂。[2]
属于三烷基膦类。[3]

應用

三(2-羧乙基)膦(TCEP)可用于:
  • 还原合成肽中的半胱氨酸残基[4]
  • RNA还原缓冲液 靶标顺序探测(SPOT)成像[5]
  • 寡核苷酸还原[6]
  • 用作线粒体分离期间的还原剂[7]

三(2-羧乙基)膦(TCEP)可用于多种下游应用,包括SDS-PAGE、质谱、半胱氨酸特异性标签标记以及含半胱氨酸化合物的修饰。它可以防止蛋白质样品的氧化,这使其成为一种实用的缓冲成分以帮助保留酶活性。[8][4][9]它已被用于谷胱甘肽的还原和测量。[10]

生化/生理作用

三(2-羧乙基)膦盐酸溶液可还原二硫键,同时保持蛋白质中的其他官能团完整。[11]
作为非硫醇还原剂,它不具有含巯基化合物固有的毒性。它能够破坏由单个二硫键连接的肉毒杆菌神经毒素B重链/轻链复合物,该复合物导致毒素的内吞并最终产生毒性。由于二硫键偶联亚基是许多毒素(如蓖麻毒素、蛇毒和所有BoNT血清型)的特有基团,因此,在毒素给药时可使用TCEP作为抢救预防措施。[12]

象形圖

Corrosion

訊號詞

Danger

危險聲明

危險分類

Eye Dam. 1 - Skin Corr. 1B

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 1

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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分析證明 (COA)

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存取文件庫

Differential labeling of free and disulfide-bound thiol functions in proteins
Seiwert B, et al.
Journal of the American Society For Mass Spectrometry, 19(1), 1-7 (2008)
Highly multiplexed quantitative mass spectrometry analysis of ubiquitylomes
Rose CM, et al.
Cell Systems, 3(4), 395-403 (2016)
E B Getz et al.
Analytical biochemistry, 273(1), 73-80 (1999-08-24)
The newly introduced sulfhydryl reductant tris(2-carboxyethyl)phosphine (TCEP) is a potentially attractive alternative to commonly used dithiothreitol (DTT). We compare properties of DTT and TCEP important in protein biochemistry, using the motor enzyme myosin as an example protein. The reductants equally
Quantitative analysis of tris (2-carboxyethyl) phosphine by anion-exchange chromatography and evaporative light-scattering detection
Tan Z, et al.
Journal of Pharmaceutical and Biomedical Analysis, 59, 167-172 (2012)
M C Gilbert Lee et al.
Journal of visualized experiments : JoVE, (87)(87), doi:10-doi:10 (2014-05-20)
Cell surface proteins, including extracellular matrix proteins, participate in all major cellular processes and functions, such as growth, differentiation, and proliferation. A comprehensive characterization of these proteins provides rich information for biomarker discovery, cell-type identification, and drug-target selection, as well

文章

Rapid trypsin digest kit yields reliable results in less than 2 hours for mass spectrometry analysis.

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.

條款

An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).

使用過濾器輔助樣品製備 (FASP) 優化 LC-MS/MS 工作流程,以進行單株抗體 adalimumab (Humira) 的低假象胰蛋白酶消化和勝肽圖譜製作。

相關內容

Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. Upon opening, may I aliquot and store this product at -20C? Or which way do you recommend storing it? Thanks.

    1 answer
    1. After opening, this product is stable for at least 1 week at room temperature under inert gas such as nitrogen or argon. The long term storage of this product has not been determined. However, the powder material, under product number 580560, reports that TCEP may be stored at -20°C in aliquots for up to 3 months. Note that this material in solution retains stability when stored at an acidic or alkaline pH. Adjustment of the pH would be required prior storage. Avoid storage at pH 7.0-8.0. Also avoid exposure to high concentrations of phosphate ions (>150 mM).

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. soultion의 shelf-life를 알고싶습니다.

    1 answer
    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Please see the link below for the product information sheet which includes recommended storage and handling information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/120/057/646547dat.pdf

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