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Merck

93350

Trizma ®

99.7% (T), puriss. p.a.

同義詞:

2-氨基-2-(羟甲基)-1,3-丙二醇, THAM, Tris 碱, 三羟甲基氨基甲烷, 氨基丁三醇

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關於此項目

線性公式:
NH2C(CH2OH)3
CAS 編號:
分子量::
121.14
Beilstein:
741883
EC號碼:
MDL號碼:
分類程式碼代碼:
12352104
PubChem物質ID:
NACRES:
NA.25

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描述

aminopeptidase substrate

等級

puriss. p.a.

化驗

99.7% (T)

形狀

crystalline

燃燒殘留物

≤0.01% (as SO4)

pH值

10.5-11.5 (25 °C, 10% in H2O)

有用的pH值範圍

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)
168-172 °C

溶解度

water: soluble (678 g/l at 20 °C)

負離子痕跡

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤5 mg/kg

正離子痕跡

Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

&lambda ;

10 % in H2O

紫外吸收

λ: 230 nm Amax: ≤0.6
λ: 260 nm Amax: ≤0.05
λ: 280 nm Amax: ≤0.04
λ: 430 nm Amax: ≤0.01

SMILES 字串

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI 密鑰

LENZDBCJOHFCAS-UHFFFAOYSA-N

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1 of 4

本產品
RDD008T4661T6066
grade

puriss. p.a.

grade

-

grade

-

grade

-

assay

≥99.7% (T)

assay

≥99.9%

assay

≥99.9% (titration)

assay

≥99.9% (titration)

Quality Level

200

Quality Level

100

Quality Level

400

Quality Level

400

form

crystalline

form

powder

form

crystalline

form

crystalline powder

pH

10.5-11.5 (25 °C, 10% in H2O)

pH

10.5-12, 7-9

pH

10.0-11.5

pH

10.0-11.5

solubility

water: soluble (678 g/l at 20 °C)

solubility

water: soluble (Water < 0.2%)

solubility

water: soluble (400 g plus 600 mL of water)

solubility

water: soluble (666 mg/ml, yielding a clear colorless solution)

一般說明

Tris 是生物化学和分子生物学中已确立的基础标准品和缓冲液。[1]可单独作为缓冲液使用,也可作为混合缓冲液配方的组分,如 Tris-EDTA (TE) 缓冲液、Tris-acetate-EDTA (TAE) 缓冲液、Tris-borate-EDTA (TBE) 缓冲液等。[2]它纯净,基本稳定,相对不吸湿,具有很高的当量。[3]

應用

已使用的 Tris 碱:
  • 在 TAE 琼脂糖凝胶的 TAE 缓冲液的制备中用于运行凝胶电泳运行 PCR 产物[4]
  • 作为 SDS-PAGE 样品缓冲液的组分[5]
  • 在制备 IEC(离子交换色谱法)缓冲液时[6]

其他說明

便于将 Trizma 产品规格与 Trizma 规格表进行比较
所有缓冲液的 pH 值都取决于温度和浓度。Tris 缓冲液,温度每降低 1°C,pH 值增加约 0.03 个单位,每稀释十倍 pH 值降低0.03-0.05 个单位。
对于精确的应用,使用经过精心校准的 pH 计和玻璃/甘汞组合电极。

法律資訊

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

相關產品

產品號碼
描述
訂價

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Quantitative determination of titin and nebulin in poultry meat by SDS-PAGE with an internal standard.
Tomaszewska-Gras J, et al.
Meat Science, 62(1), 61-66 (2002)
a-Synuclein Aggregation Monitored by Thioflavin T Fluorescence Assay.
Wordehoff M and Hoyer W
Bio-protocol, 8(14) (2018)
In vivo Analysis of Cyclic di-GMP Cyclase and Phosphodiesterase Activity in Escherichia coli Using a Vc2 Riboswitch-based Assay.
Liu Y, et al.
Journal of Bacteriology (2017)
Tri (hydroxymethyl) aminomethane as Acidimetric Standard.
Fossum J, et al.
Analytical Chemistry, 23(3), 491-493 (1951)
Preparation of Buffers for Use in Enzyme Studies.
Gomori G
Methods in Enzymology, 1, 138-146 (1955)

相關內容

Trizma Base Specification Comparison

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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