Each vial contains a minimum of 1 mg of 5-Azacytidine. The exact quantity may vary from lot to lot and is reported in the Certificate of Analysis. This product is tested for solubility at 5 mg/mL in a 1:1 mixture of acetic acid and water. For use in cell culture, the material may be prepared at 1.2 mg in 10 mL of sterile medium without supplements. The stock solution should be stored in aliquots at -20°C. Avoid repeated freeze/thaw cycles. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/a1287#product-documentation
A1287
5-阿扎胞苷
Hybri-Max™, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma
同義詞:
4-氨基-1-(β-D-呋喃核糖基)-1,3,5-三嗪-2(1H)-酮, 阿扎胞苷
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經驗公式（希爾表示法）:
C8H12N4O5
CAS 編號:
分子量：:
244.20
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
EC Number:
206-280-2
Beilstein/REAXYS Number:
620461
MDL number:
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產品名稱
5-阿扎胞苷, Hybri-Max™, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma
InChI key
NMUSYJAQQFHJEW-KVTDHHQDSA-N
InChI
1S/C8H12N4O5/c9-7-10-2-12(8(16)11-7)6-5(15)4(14)3(1-13)17-6/h2-6,13-15H,1H2,(H2,9,11,16)/t3-,4-,5-,6-/m1/s1
SMILES string
NC1=NC(=O)N(C=N1)[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O
biological source
synthetic (organic)
grade
Hybri-Max™
sterility
γ-irradiated
product line
BioXtra
form
lyophilized powder
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
mp
226-232 °C (dec.) (lit.)
solubility
acetic acid: water (1:1): 5 mg/mL, clear, colorless
antibiotic activity spectrum
viruses
mode of action
DNA synthesis | interferes
storage temp.
−20°C
Quality Level
Gene Information
human ... DNMT1(1786), DNMT3A(1788)
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本產品 | PHR1911 | 1045520 | A3656 |
|---|---|---|---|
| form lyophilized powder | form powder | form - | form powder |
| technique(s) cell culture | hybridoma: suitable | technique(s) - | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 300 | Quality Level - | Quality Level 200 |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. - | storage temp. - |
| solubility acetic acid: water (1:1): 5 mg/mL, clear, colorless | solubility - | solubility - | solubility acetic acid: water (1:1): 50 mg/mL |
| biological source synthetic (organic) | biological source - | biological source - | biological source - |
Application
用于研究DNA掺入分子对DNA甲基化过程的影响。
Biochem/physiol Actions
5-氮杂胞苷是脱氧胞苷类似物和去甲基化剂。[3]它可作为急性骨髓性白血病的潜在抗肿瘤药。[4]5-氮杂胞苷通过抑制DNA甲基化来激活被抑制的基因。[5]5-氮杂胞苷还通过改变RNA功能和稳定性来影响蛋白质合成。[4]
一种强效生长抑制剂及细胞毒素剂，抑制 DNA 甲基转移酶，一种基因表达、基因激活和沉默的重要调节机制。
强效生长抑制剂及细胞毒素剂，抑制 DNA 甲基转移酶。
General description
化学结构：核苷
Preparation Note
10mL 无菌细胞培养基可复原一小瓶本品。储备液足以制备 500mL 培养基。最终工作浓度：10μM 5-氮杂胞嘧啶核苷。
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1B - STOT RE 1
存儲類別/等級
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
DNA methyltransferase inhibitors for cancer therapy
Epigenetics, 5(3), 265-290 (2015)
5-azacytidine treatment reorganizes genomic histone modification patterns
Komashko VM and Farnham PJ
Epigenetics, 5(3), 229-240 (2010)
S Massah et al.
BMC genetics, 21(1), 3-3 (2020-01-17)
DNA methylation is an epigenetic modification that mainly repress expression of genes essential during embryogenesis and development. There are key ATPase-dependent enzymes that read or write DNA methylation to remodel chromatin and regulate gene expression. Structural maintenance of chromosome hinge
Manuel Luís Orta et al.
Nucleic acids research, 41(11), 5827-5836 (2013-04-24)
5-Aza-2'-deoxycytidine (5-azadC) is a DNA methyltransferase (DNMT) inhibitor increasingly used in treatments of hematological diseases and works by being incorporated into DNA and trapping DNMT. It is unclear what DNA lesions are caused by 5-azadC and if such are substrates
5-Azacytidine treatment sensitizes tumor cells to T-cell mediated cytotoxicity and modulates NK cells in patients with myeloid malignancies
Gang AO, et al.
Blood Cancer Journal, 4(3), e197-e197 (2014)
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What is the vial content of A1287, what is the solubility, and what are to storage conditions for the prepared stock solution?
1 answer-
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What is the concentration of Product A1287, 5-Azacytidine - Hybri-Max™?
1 answer-
The working concentration is 10 μM, so the 50X stock is 500 μM.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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