This is not a species specific antigen, it is a slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. It has multi-species reactivity as it is a highly conserved region of the protein among species.
A5441
Anti-β-Actin (ACTB) Antibody
mouse monoclonal, AC-15
Monoclonal Anti-β-Actin antibody produced in mouse
同義詞:
单克隆抗-β-Actin
跳轉至
產品名稱
单克隆抗 β-肌动蛋白抗体 小鼠抗, clone AC-15, ascites fluid
生物源
mouse
共軛
unconjugated
抗體表格
ascites fluid
抗體產品種類
primary antibodies
無性繁殖
AC-15, monoclonal
分子量
antigen 42 kDa
包含
15 mM sodium azide
物種活性
sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
應無反應活性
Dictyostelium discoideum
技術
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:1,000-1:2,000 using cultured human or chicken fibroblasts
western blot: 1:5,000-1:10,000 using cultured human or chicken fibroblast cell extracts
同型
IgG1
UniProt登錄號
應用
research pathology
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
尋找類似的產品？ 前往 產品比較指南
1 of 4
本產品 | F3777 | T5168 | T4026 |
|---|---|---|---|
| conjugate unconjugated | conjugate FITC conjugate | conjugate unconjugated | conjugate unconjugated |
| clone AC-15, monoclonal | clone 1A4, monoclonal | clone B-5-1-2, monoclonal | clone TUB 2.1, monoclonal |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| Gene Information human ... ACTB(60) | Gene Information human ... ACTA2(59) | Gene Information human ... TUBA4A(7277) | Gene Information human ... TUBB(203068), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383) |
| antibody form ascites fluid | antibody form purified from hybridoma cell culture | antibody form ascites fluid | antibody form ascites fluid |
| species reactivity sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig | species reactivity human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake | species reactivity mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat | species reactivity human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster |
一般說明
在鸡砂囊超薄组织冷冻切片的染色中，抗体对致密体和由结蛋白和膜相关的致密斑所占据的纵向通道连接的连续致密体进行了标记。它不会对成人的心脏和骨骼肌进行染色，除非是受到了非肌肉细胞样本的污染，或是使用了胚胎组织。
抗-β-肌动蛋白单克隆抗体（小鼠IgG1亚型）来自于免疫小鼠骨髓瘤细胞和脾细胞相互融合而产生的AC-15杂交瘤细胞。肌动蛋白是最保守的真核蛋白之一，在哺乳动物和鸟类中至少表达6种异构体。其中四种代表肌肉组织的分化标志物，而两种在几乎所有细胞中都有所发现。现有三种α-肌动蛋白（α-骨骼肌、α-心脏和 α-平滑肌），一种β-肌动蛋白（β-非肌肉）和两种γ-肌动蛋白（γ-平滑肌和γ-非肌肉）。肌动蛋白异构体显示>90%的整体序列同源性，但在其18个NH2-末端残基中只有5060%的同源性。肌动蛋白的NH2-末端区域似乎是一个主要的抗原区域，可能参与了肌动蛋白与肌球蛋白等其他蛋白的相互作用。该抗体可用于丙酮固定的冷冻切片、EM制品和显微注射实验的染色。B5、乙醇、methacam或Bouin′s溶液可用作固定剂。抗体所识别的表位对福尔马林固定和石蜡包埋具有抗性。
免疫原
略微修改的β-细胞质肌动蛋白N末端肽，Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys，偶联至KLH。
應用
单克隆小鼠抗肌动蛋白抗体被用作一种上样对照，用于对来自大鼠背根神经节共培养物的免疫沉淀蛋白进行western blot分析。
单克隆小鼠抗肌动蛋白抗体被用作大鼠肝脏蛋白裂解液western blot分析的一种上样对照。
小鼠源抗-β-肌动蛋白单克隆抗体可用于：
- 免疫荧光染色
- 免疫印迹
- 免疫组织化学
- 用作蛋白芯片的对照品
生化／生理作用
抗β-肌动蛋白单克隆抗体能够识别位于肌动蛋白β-亚型N-端上的表位。在免疫印迹（42 kDa处的条带）、培养细胞的免疫荧光染色和免疫组织化学分析中，该抗体能特异性标记各种组织和物种中的β-肌动蛋白。
肌动蛋白和肌球蛋白是许多类型细胞的组成部分，参与运动、胞质分裂、细胞质流动、分泌和吞噬等诸多细胞过程。不同物种和组织来源细胞中的肌动蛋白的免疫学和生理特性非常相似。
外觀
以含有15mM叠氮化钠作为防腐剂的腹水形式提供。
其他說明
想要查看肌动蛋白抗体选择指南，请访问 www.sigmaaldrich.com/actin。
免責聲明
除非在我们的产品目录或产品随附的其他公司文档中另有说明，我们的产品仅供研究使用并不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途、任何类型的食用或应用于人或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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文章
Actin isoforms are muscle tissue markers; six isoforms expressed, four for muscle differentiation, two ubiquitous.
抗體與特定抗原結合，產生專屬的抗體抗原複合物。了解這種結合的本質及其作為分子標籤的研究用途。
了解單克隆抗體與多克隆抗體的差異，包括抗體的產生方式、克隆數量和抗體格式。
免疫螢光法使用抗體結合的螢光分子來進行蛋白質定位、修改確認及蛋白質複合物可視化。
條款
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.
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species of antigen
1 answer-
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Why do I have 3 bands on my western blot when using Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
1 answer-
While actin should run as a 42 kDa band when detected by Western blotting, there are recent observations that cleavage products can be seen in cells undergoing apoptosis. Initial cleavage gives a band at 41 kDa, which may appear as a doublet on the blot. In addition, further cleavage results in a major band at 30 kDa and a small band at 14 kDa which have been shown to be cleavage products due to the action of interleukin 1 -converting enzyme. This may explain the detection of smaller bands when blotting with this antibody. This banding pattern may be detected with other actin antibodies as well. Kayalar, C., et al., Proc. Natl. Acad. Sci., USA, 93:2234-2238, (1996).
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Can I use Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse, to detect mouse beta actin?
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This antibody will detect mouse beta actin. When used for western blotting, the secondary antibody may react with mouse IgG from the sample resulting with a bands at 50 kDa and 25 kDa (reducing conditions) or approximately 150 kDa (non-reducing conditions). It is best to use a directly conjugated antibody for immunohistochemistry or immunofluorescent assays. We offer Product numbers A3854, peroxidase conjugated.
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What is the antibody concentration of Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
1 answer-
This antibody is ascities fluid. The amount of protein present and the amount of IgG1 present is indicated on the Lot-specific Certificate of Analysis.
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What is the molecular weight of Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
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Beta Actin has a molecular weight of 42 kDa.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What IgG light chain (kappa or lambda) is associated with Clone AC-15 for Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
1 answer-
We have not determined the light chain used by monoclonal antibody AC-15. 95% of mouse antibodies have kappa light chains.
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What peroxidase-conjugated secondary antibody should I use with Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
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We recommend using Product No. A9044, rabbit anti-mouse IgG, peroxidase conjugate as a secondary antibody. We also offer this same antibody directly conjugated to peroxidase as Product No. A3854.
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