Name: Human Collagen Type IV
Brand: SIGMA

產品名稱

胶原蛋白来源于人类胎盘, Bornstein and Traub Type IV, powder, BioReagent, suitable for cell culture

biological source

human placenta

product line

BioReagent

form

powder

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

impurities

HIV, hepatitis B and hepatitis C, none detected

solubility

acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)

UniProt accession no.

P02462
P08572
P29400
P53420
Q01955
Q14031

binding specificity

Peptide Source: Collagen Type IV

storage temp.

−20°C

Quality Level

200

Gene Information

human ... COL4A1(1282), COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)

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本產品	H4417	C7521	C8374
Sigma-Aldrich C5533 胶原蛋白来源于人类胎盘	Sigma-Aldrich H4417 胶原蛋白来源于人类胎盘	Sigma-Aldrich C7521 胶原蛋白来源于人类胎盘	Sigma-Aldrich C8374 胶原来源于人类胎盘
biological source human placenta	biological source human placenta	biological source human placenta	biological source human placenta
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) cell culture \| stem cell: suitable
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form powder	form solution	form powder	form powder
packaging pkg of 5 mg	packaging -	packaging -	packaging -
binding specificity Peptide Source: Collagen Type IV	binding specificity -	binding specificity -	binding specificity -

Application

人胎盘来源胶原蛋白已用于：

溶解细胞外基质，以进行表面官能化。[1]
诱导内皮细胞[2]和大鼠多能细胞分化。[3]
作为细胞迁移/侵袭测定的粘附剂。[4]
评估奎宁对气道上皮细胞的生物电效应。
研究工程化慢病毒裂解肽的选择性毒性。
颗粒聚集测定，快速检测金黄色葡萄球菌上的纤连蛋白、纤维蛋白原和胶原受体。
鉴定人肺肿瘤相关抗原过程中进行的镁依赖性胶原结合测定。

Biochem/physiol Actions

IV型胶原蛋白可维持细胞分裂和生长。[5]胶原蛋白分泌异常会影响基底膜的形成。[6]IV型胶原蛋白α1 (COL4A1)基因在胃癌中过表达。[7]COL4A1的突变会导致成人和儿童脑出血。[8]COL4A1和COL4A2突变可导致脑血管疾病。[9]经证IV型胶原蛋白在血管生成、神经疾病和肿瘤转移中具有关键作用。
在发育过程中，胶原蛋白IV普遍分布在基底膜(BM)中。在成熟过程中，该网状纤维以显著的组织特异性方式被部分替换，从而定义BM结构和功能。胶原蛋白IV可以结合血小板、肝细胞、角质形成细胞、内皮细胞、肾小球膜细胞、胰腺细胞以及一些肿瘤细胞。
自身免疫性疾病Goodpasture综合征中的组织损伤是由靶向胶原蛋白IV α3链的致病性自身抗体引起的。COL4A5基因突变与Alport综合征有关。

Disclaimer

该产品应在-20°C下干燥保存，在此条件下可保持活性3年。

General description

所有胶原蛋白分子均由以三螺旋构象排列的三条肽链组成，其一级结构主要以三个氨基酸作为重复基序，即每隔两个氨基酸是一个甘氨酸残基，其前面的两个位置通常为脯氨酸或4-羟基脯氨酸。IV型胶原仅存在于基底膜中，并含有多达六条基因不同的a链。IV型胶原位于基底膜上，是网络形成蛋白质。它由六个不同的基因编码：IV型胶原蛋白α1 (COL4A1)、COL4A2、COL4A3、COL4A4、COL4A5和COL4A6。COL4A1和COL4A2基因都位于人类染色体13q34上。这两个基因编码的蛋白质具有45％的同源性。其中含有共有的非胶原（NC1）结构域、三螺旋胶原结构域和Gly-Xaa-Yaa重复序列。[9]COL4A3和COL4A4基因都位于人类染色体2q36.3上。[10]在组织中，IV型胶原蛋白以原胶原蛋白的形式存在。[5]

Other Notes

胶原蛋白被分为许多种结构和遗传性不同的类型。我们使用 Bornstein和Traub提出的系统命名法。小心不要混淆Sigma命名系统和通用的胶原蛋白分类类型。

Preparation Note

本品为冻干粉末，能够以1 mg/mL的浓度重悬于无菌的.5 M乙酸、PBS或水中。 PBS溶液在-20°C下可稳定保存至少一年。它是利用改良的Niyibizi胃蛋白酶提取方法进行制备的

存儲類別/等級

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number

0000446974

0000347798

0000282901

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Genetic Variations of the COL4A1 Gene and Intracerebral Hemorrhage Risk: A Case-Control Study in a Chinese Han Population

Lin S, et al.

World Neurosurgery, 112, e527-e533 (2018)

Genotype-phenotype correlations in pathology caused by collagen type IV alpha 1 and 2 mutations

Jeanne M and Gould DB

Matrix Biology, 57, 29-44 (2017)

Color Atlas of Dermatoimmuno Histocytology, 62-62 (1989)

A cell migration device that maintains a defined surface with no cellular damage during wound edge generation

Doran MR, et al.

Lab on a chip, 9(16), 2364-2369 (2009)

Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis

Banushi B, et al.

Nature Communications, 7, 12111-12111 (2016)

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什麼是細胞外基質？

細胞外基質 (ECM) 由細胞分泌，包圍在組織中。

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癌症幹細胞培養基、球形板和癌症幹細胞標記，以培養和表徵 CSC 群。

Attachment Factors for Three-dimensional Cell Culture

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

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Questions

1–3 of 3 Questions

6 months ago

I want to know its molecular weight.

1 answer
1. Technical Support
  
  6 months ago
  
  This product is a collagen Type IV, which contains up to 6 distinct a-chains. The molecular weight may be quite variable and is not determined on a lot-to-lot basis. Historical investigations reveal 3 bands on SDS-PAGE gels with corresponding molecular weights as follows:
  Band 1: 102 - 104 kDa
  Band 2: 157 - 162 kDa
  Band 3: 178 - 181 kDa
  In addition, the Merck Index reports a molecular weight of 130 kDa and the CRC reports a molecular weight of 95 kDa or greater.
  (Merck Index, 11th ed., p. 387, #2476 / CRC-Collagen, vol. I, Biochemistry, 140 (1988))
  
  Helpful?
Anonymous

2 years ago

Hi, I have several questions about collagen coating. 1.when we dissolve the collagenIV powder in 0.5M acetic acid, can it be stored at -30 degree? 2. When we dilute the collagen stock with 0.25% acetic acid, how long can i store it at 4 degree?

1 answer
1. Technical Support
  
  2 years ago
  
  Solutions of 1 -2 mg/mL collagen in 0.5M acetic acid should be stable for at least 1 year at -20°C.
  Collagen solutions stored at 2-8°C should remain stable only for a few days up to 1 week.
  
  Helpful?
Anonymous

2 years ago

Goodmorning, I noticed that in the upper instructions it is stated to use acetic acid but below it seems to be also possible to use water/PBS. What holds true? And is the powder itself stable?

1 answer
1. Technical Support
  
  2 years ago
  
  As mentioned on the product page under 'Preparation Note'' in the DESCRIPTION section, this lyophilized powder can be reconstituted in sterile 0.5 M acetic acid, water, or PBS to a concentration of 1 mg/mL. The product is tested for solubility in acetic acid at 1 mg/mL. The powder is not assigned an expiration date. It is assigned a retest schedule of every 3 years and is quite stable if stored properly.
  Please see the link below to review the product data sheet for more information:
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/259/550/c5533pis.pdf
  
  Helpful?

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Anonymous

4 years ago

Functional protein for primary cell culture

I ordered this product as recommended by a protocol for use in a plate coating buffer for primary cell culture. It is a functional protein and supports epithelial cell growth.

Recommends this product

Yes

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Human Collagen Type IV

from human placenta, powder, suitable for cell culture

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