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Merck

C7698

环己酰亚胺,大包装

from microbial, ≥94% (TLC)

同義詞:

3- [2-(3,5-二甲基-2-氧代环己基)-2-羟乙基]戊二酰亚胺, 放线菌酮, 纳拉霉素A

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經驗公式(希爾表示法):
C15H23NO4
CAS 編號:
分子量::
281.35
UNSPSC Code:
12352111
NACRES:
NA.77
PubChem Substance ID:
EC Number:
200-636-0
Beilstein/REAXYS Number:
88868
MDL number:

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產品名稱

环己酰亚胺,大包装, from microbial, ≥94% (TLC)

InChI

1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1

SMILES string

[H][C@]1(C[C@@H](C)C[C@H](C)C1=O)[C@H](O)CC2CC(=O)NC(=O)C2

InChI key

YPHMISFOHDHNIV-FSZOTQKASA-N

biological source

microbial

assay

≥94% (TLC)

form

powder

color

white to off-white

solubility

ethanol: soluble, clear to hazy

antibiotic activity spectrum

fungi
yeast

mode of action

protein synthesis | interferes

storage temp.

2-8°C

Quality Level

Gene Information

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1 of 4

本產品
23976546401239763-M
assay

≥94% (TLC)

assay

-

assay

≥95% (HPLC)

assay

≥90% (by assay)

form

powder

form

solution

form

-

form

powder

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

room temp

solubility

ethanol: soluble, clear to hazy

solubility

DMSO: soluble

solubility

H2O: slightly soluble

solubility

-

color

white to off-white

color

-

color

-

color

white to brown

Application

  • 在酵母菌株中,环己酰亚胺已被用作环己酰亚胺追踪实验中的蛋白质合成抑制剂。[1]
  • 它已被用于抑制哺乳动物细胞中的翻译。[2][3]
  • 它已被用于抑制真菌生长。[4]

Biochem/physiol Actions

作用机制:真核生物中的翻译抑制导致细胞生长停滞和细胞死亡。CHX被广泛用于抗CHX的酵母和真菌菌株的选择、蛋白质合成的受控抑制以检测短寿命蛋白质和蛋白质表达的超诱导作用、以及诱导凋亡或促进通过死亡受体诱导的凋亡。

活性谱:具有抑制酵母和真菌如念珠菌、曲霉、酵母菌和青霉的活性

Features and Benefits

  • 适用于多种研究应用的优质抗生素
  • 常用于细胞生物学和生物化学研究

General description

环己酰亚胺,也称放线菌酮,是一种戊二酰亚胺娄抗生素,通常来源于灰色链霉菌。它可用作真核细胞中蛋白质生物合成的强效抑制剂,其机理是破坏翻译中的转运步骤,从而有效阻止翻译延伸(translational elongation)。在微生物学领域,作为酵母和真菌抗性菌株的选择剂它起到关键作用,在受控实验环境中非常重要。

在细胞生物学和生物化学研究领域,环己酰亚胺体现出与诱导细胞凋亡有关的双重特性,即根据细胞类型不同诱导或抑制细胞凋亡。快速且可逆的作用令其成为研究细胞过程和测定蛋白质半衰期的理想选择。环己酰亚胺也在生物医学研究中得到广泛应用,它抑制体外(生物体外)研究的真核细胞中的蛋白质合成,去除环己酰亚胺则可快速逆转其作用。这一特性使环己酰亚胺成为细胞生物学、生物医学和生物化学研究的最佳选择,为实验带来了精准的控制和多功能性。

Other Notes

保存于密闭容器内,置于干燥通风处。置于干燥处保存。
如需了解生化试剂系列的更多信息,请填写此表

Preparation Note

密闭。干燥。于通风良好处保存。上锁保存,或保存在仅对有资格或授权人员
开放的区域。

signalword

Danger

Hazard Classifications

Acute Tox. 2 Oral - Aquatic Chronic 2 - Muta. 2 - Repr. 1B

存儲類別/等級

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Potential pathogenic bacteria in metalworking fluids and aerosols from a machining facility.
Perkins SD and Angenent LT
FEMS Microbiology Ecology, 74, 643-643 (2010)
Nonsense-mediated mRNA decay (NMD) blockage promotes nonsense mRNA stabilization in protein 4.1R deficient cells carrying the 4.1R Coimbra variant of hereditary elliptocytosis.
Moriniere M et al.
Blood Cells, Molecules and Diseases, 45, 284-284 (2010)
Quality control of a transcriptional regulator by SUMO-targeted degradation.
Wang Z and Prelich G
Molecular and Cellular Biology, 29, 1694-1694 (2009)
Zhen Zhang et al.
Frontiers in endocrinology, 5, 206-206 (2014-12-19)
O-linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification involving an attachment of a single β-N-acetylglucosamine moiety to serine or threonine residues in nuclear and cytoplasmic proteins. Cellular O-GlcNAc levels are regulated by two enzymes: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), which
Jianzhen Li et al.
Biology of reproduction, 100(2), 468-478 (2018-11-13)
The role of androgenic steroids on ovarian development has attracted much attention in recent years, but the molecular mechanism is still largely unknown. In this study, using zebrafish as a model, we found that the trace metal zinc mediates the

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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