C8302

4-氯-1-萘酚溶液

Name: 4-氯-1-萘酚 溶液
Brand: SIGMA

For HRP detection on Western Blots

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CAS 編號:

604-44-4

MDL號碼:

MFCD00003974

分類程式碼代碼:

12352200

PubChem物質ID:

24893047

NACRES:

NA.52

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等級

Molecular Biology

品質等級

200

形狀

liquid

儲存溫度

2-8°C

SMILES 字串

Oc1ccc(Cl)c2ccccc12

InChI

1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H

InChI 密鑰

LVSPDZAGCBEQAV-UHFFFAOYSA-N

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本產品	N7660	185507	N2125
Sigma-Aldrich C8302 4-氯-1-萘酚溶液	Sigma-Aldrich N7660 4-硝基苯酚溶液	Sigma-Aldrich 185507 2-萘酚	Sigma-Aldrich N2125 萘酚 AS-BI 磷酸盐
grade for molecular biology	grade -	grade -	grade -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form liquid	form liquid	form powder or flakes	form powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. -	storage temp. −20°C

一般說明

4-氯-1-萘酚是一种可产生有色沉淀反应中的HRP底物。它通常用于western blotting中的比色检测和蛋白可视化。

應用

适用于在western blottin中观察蛋白-过氧化物酶耦合物。

生化／生理作用

诸如HRP之类的酶可将4-氯-1-萘酚转化为印迹上肉眼轻易可见的紫色沉淀物。

其他說明

0.48 mM 4-氯-1-萘酚、50 mM Tris-HCl、0.2 M NaCl以及17%甲醇。

该产品含有0.48 mM 4-氯-1-萘酚、50 mM Tris-HCl和0.2 M NaCl以及17%甲醇。

P7170

丽春红S染液(零售包装) 溶液, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

P7059

磷酸盐缓冲溶液, 10× PBS for Western blots and IP

RPROB

Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels

W0138

Western Blocker^™溶液, for HRP detection systems

SDS7B2

Prestained Molecular Weight Marker, mol wt 26,600-180,000 Da

C1992

ColorBurst ^™ 电泳标记, mol wt 8,000-220,000 Da

GERPN2209

ECL^™ Western Blotting Detection Reagents, Cytiva RPN2209

象形圖

GHS08,GHS07

訊號詞

Danger

危險聲明

H302 + H312 + H332,H370

防範說明

P260 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P311

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - STOT SE 1

標靶器官

Eyes,Central nervous system

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類（WGK）

WGK 2

閃點（°F）

163.9 °F - closed cup

閃點（°C）

73.3 °C - closed cup

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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存取文件庫

Enhanced monoterpene emission in transgenic orange mint (Mentha × piperita f. citrata) overexpressing a tobacco lipid transfer protein (NtLTP1).

Hwan-Su Hwang et al.

Planta, 252(3), 44-44 (2020-09-03)

Overexpression of the tobacco lipid transfer protein (NtLTP1) gene in transgenic orange mint resulted in enhanced accumulation of monoterpenes in the cavity of head cells of glandular trichomes, which resulted in enhanced emission of monoterpenes from transgenic orange mints. Plants

Role of PKCζ-NADPH oxidase signaling axis in PKCα-mediated Giα2 phosphorylation for inhibition of adenylate cyclase activity by angiotensin II in pulmonary artery smooth muscle cells.

Animesh Chowdhury et al.

Cell biology international, 44(5), 1142-1155 (2020-01-23)

We sought to determine the mechanism by which angiotensin II (AngII) inhibits isoproterenol induced increase in adenylate cyclase (AC) activity and cyclic adenosine monophosphate (cAMP) production in bovine pulmonary artery smooth muscle cells (BPASMCs). Treatment with AngII stimulates protein kinase

文章

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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4-氯-1-萘酚 溶液