The solution storage and stability of this product have not been established. However, the activated or nicked version of this material, product D4522, notes that Solutions of DNA have been stored successfully for several months at 4 °C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. Please see the link below to review additional information available in the datasheet for the D4522:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/630/619/d4522pis.pdf
D4764
脱氧核糖核酸 来源于小牛胸腺
Genomic, unsheared
同義詞:
DNA 来源于小牛胸腺, 小牛胸腺DNA, 胸腺核酸
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本產品 | D8899 | D4522 | D8515 |
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade for molecular biology | grade - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt >20 kb | mol wt ~50 kb | mol wt - | mol wt - |
一般說明
从雄性和雌性小牛的胸腺分离的高质量双链模板DNA。
應用
小牛胸腺DNA非常适合用于作为DNA聚合酶测定的底物、长片段的扩增、以及作为沉淀时的载体DNA。
生化／生理作用
42% GC；Tm = 87 °C.
％GC是指G/C碱基对的百分比。Tm是热变性曲线中点处的温度。
％GC是指G/C碱基对的百分比。Tm是热变性曲线中点处的温度。
特點和優勢
•在37°C温育16小时后未检测到核酸酶活性
•高质量的模板DNA
•高质量的模板DNA
準備報告
将DNA溶解在分子生物级水中并轻轻摇动过夜，使终浓度为1mg/ml。
阴离子交换色谱纯化，用含1mM氯化钠、1mM EDTA和1mM Trizma HCl的溶液（pH 7.5）透析，并以约2 A260 单位/mL的浓度冻干。约20 260单位/mg DNA。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
Roel J H Custers et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 28(4), 429-435 (2009-11-04)
The purposes of the present study were to explore the surgical possibilities for replacement of the medial tibial plateau by a metallic implant in a large animal model and to examine the implications for the opposing cartilage. In six goats
A Châtel et al.
Ecotoxicology (London, England), 20(8), 1727-1740 (2011-06-10)
In the present study, we analyzed the effects of two major pollutants of the environment, tributyltin (TBT) and water-accommodated fraction (WAF) of diesel oil, on MAP kinase activation, apoptosis induction and DNA damage, in the marine sponge Suberites domuncula. Our
Nathalie W D Jansen et al.
Arthritis and rheumatism, 56(1), 199-207 (2006-12-30)
Joint bleeding, or hemarthrosis, leads in time to severe joint damage. This study was carried out to test the in vitro thresholds of exposure time and concentration that lead to irreversible joint damage, to add to the discussion on the
José Luis García-Giménez et al.
Journal of inorganic biochemistry, 121, 167-178 (2013-02-07)
Ternary copper(II) complexes [Cu(NST)2(phen)] (1) and [Cu(NST)2(NH3)2]·H2O (2) [HNST=N-(4,5-dimethylthiazol-2-yl)naphthalene-1-sulfonamide] were prepared and characterized by physico-chemical techniques. Both 1 and 2 were structurally characterized by X-ray crystallography. The crystal structures show the presence of a distorted square planar CuN4 geometry in
Sutha Shobana et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 107, 117-132 (2013-02-19)
Equilibrium studies of Ni(II), Cu(II) and Zn(II) mixed ligand complexes involving a primary ligand 5-fluorouracil (5-FU; A) and imidazoles viz., imidazole (him), benzimidazole (bim), histamine (hist) and L-histidine (his) as co-ligands(B) were carried out pH-metrically in aqueous medium at 310±0.1K
相關內容
Datasheet
Datasheet
Instructions
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What are the storage conditions once the DNA is resuspended? Should I keep the DNA at -20ºC or -80ºC?
1 answer-
Helpful?
-
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how much is 1 unit (i.e. in ug or mg)?
1 answer-
One unit, based on the A260 value, is approximately 50 µg. The general conversion formula is 20 units per milligram. The 0.25 UN package size will contain approximately 12.5 µg, the 1 jUN package size will contain approximately 50 µg, and the 5UN package size will contain approximately 250 µg.
For additional product information, please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/289/542/d4764dat.pdfHelpful?
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