P2287

TWEEN ^® 20

Name: TWEEN <SUP>®</SUP> 20
Brand: SIGMA

viscous liquid, suitable for cell culture

同義詞:

聚山梨酯 20, 聚氧乙烯山梨糖醇酐单月桂酸酯, 聚氧乙烯脱水山梨醇单月桂酸酯

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CAS 編號:

9005-64-5

MDL號碼:

MFCD00165986

分類程式碼代碼:

12161900

NACRES:

NA.21

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描述

non-ionic

形狀

viscous liquid

分子量

~1228

成份

lauric acid, ≥40% (balance primarily myristic, palmoyic, and stearic acids)

濃度

≥40.0%

技術

cell culture | mammalian: suitable

折射率

n20/D 1.468 (lit.)

CMC

0.06 mM (20-25°C)

轉變溫度

cloud point 76 °C

溶解度

water: soluble (Water solubility: 0,0002 g/l at 20 °C - OECD Test Guideline 105)

密度

1.095 g/mL at 25 °C (lit.)
1.1 g/cm³ at 25 °C (lit.)

HLB

16.7

應用

(ELISA and immunoassays)

SMILES 字串

CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

InChI 密鑰

HMFKFHLTUCJZJO-UHFFFAOYSA-N

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本產品	P9416	P7949	93773
Sigma-Aldrich P2287 TWEEN ^® 20	Sigma-Aldrich P9416 TWEEN ^® 20	Sigma-Aldrich P7949 TWEEN ^® 20	Sigma-Aldrich 93773 TWEEN ^® 20
technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) blocking: suitable, cell culture \| mammalian: suitable, hybridization: suitable, protein purification: suitable, transformation: suitable	technique(s) DNA extraction: suitable, RNA extraction: suitable, cell culture \| mammalian: suitable, protein purification: suitable
form viscous liquid	form viscous liquid	form viscous liquid	form viscous liquid
Quality Level 300	Quality Level 300	Quality Level 300	Quality Level 200
solubility water: soluble	solubility water: 100 mg/mL, clear, colorless to yellow	solubility -	solubility -
density 1.095 g/mL at 25 °C (lit.), 1.1 g/cm³ at 25 °C (lit.)	density 1.095 g/mL at 25 °C (lit.)	density 1.095 g/mL at 25 °C (lit.)	density 1.11 g/mL at 20 °C, 1.095 g/mL at 25 °C (lit.)
description non-ionic	description non-ionic	description non-ionic	description non-ionic

一般說明

吐温20是聚氧乙烯山梨醇酯，属于聚山梨醇家族。这是一种分子量为1225道尔顿的非离子洗涤剂，认为其含20个环氧乙烷单位、1个山梨醇和1个月桂酸为主要脂肪酸。环氧乙烷亚基提供了表面活性剂的亲水性，而碳氢化合物链提供疏水环境。山梨醇形成连接环氧乙烷聚合物的骨架环。[1]

應用

吐温20已可用于原位杂交的染色缓冲液中。[2]在荧光共振共振能量转移(FRET)实验样品制备过程中，它也被用来防止蛋白质对盖玻片的非特异性吸附。[3]

吐温20是一种广泛应用于生化领域的非离子去污剂。它被用作稳定的水包油乳液的乳化剂。吐温20已可用于膜的预提取，以去除周围蛋白(用于提取膜结合蛋白的比例为2%)。
吐温20在基于膜的免疫分析中已被用作一种封闭剂，其常用浓度为0.05%。吐温20可用于裂解哺乳动物细胞，使用浓度为0.005至0.5%。

非离子洗涤剂

準備報告

吐温20可混溶于水（100mg/mL），得到透明的黄色溶液。它也可以与酒精，二恶烷，乙酸乙酯混溶。吐温20不溶于液体石蜡和固定油脂。

法律資訊

TWEEN is a registered trademark of Croda International PLC

免責聲明

吐温20在高压灭菌下可能不稳定，特别是在含金属阳离子的缓冲溶液中。如果没有对性能的变化进行测试，则不建议使用高压灭菌。吐温20对热敏感，放置于升高的温度中其颜色会变深。据报道，聚山梨醇酯与碱、重金属盐、酚和单宁酸不相容。它们可能会降低许多防腐剂的活性。聚山梨醇酯水溶液在贮存过程中发生自氧化反应，光、温度升高和硫酸铜催化其变化。

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

527.0 °F - Pensky-Martens closed cup

閃點（°C）

275 °C - Pensky-Martens closed cup

個人防護裝備

Eyeshields, Gloves

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分析證明 (COA)

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Versatile wetting measurement of microplate wells.

Enoch Ming Wei Ng et al.

The Review of scientific instruments, 87(11), 115107-115107 (2016-12-03)

A method to measure the contact angle, which is indicative of wetting, using small liquid volumes dispensed directly on microplate wells is described and demonstrated. Experiments with enhanced green protein samples of volumes 4.4-6 μl showed no measured variance in

Adverse morphological development in embryonic zebrafish exposed to environmental concentrations of contaminants individually and in mixture.

Kinch CD, et al.

Aquatic Toxicology (Amsterdam, Netherlands), 175, 286-298 (2016)

Conformations of a metastable SH3 domain characterized by smFRET and an excluded-volume polymer model.

Mazouchi A, et al.

Biophysical Journal, 110(7), 1510-1522 (2016)

Oral perfluorooctane sulfonate (PFOS) lessens tumor development in the APCmin mouse model of spontaneous familial adenomatous polyposis.

Jeffrey Wimsatt et al.

BMC cancer, 16(1), 942-942 (2016-12-09)

Colorectal cancer is the second most common cause of cancer deaths for both men and women, and the third most common cause of cancer in the U.S. Toxicity of current chemotherapeutic agents for colorectal cancer, and emergence of drug resistance

Adverse morphological development in embryonic zebrafish exposed to environmental concentrations of contaminants individually and in mixture.

Cassandra D Kinch et al.

Aquatic toxicology (Amsterdam, Netherlands), 175, 286-298 (2016-04-24)

Exposure to environmental contaminants has been linked to developmental and reproductive abnormalities leading to infertility, spontaneous abortion, reduced number of offspring, and metabolic disorders. In addition, there is evidence linking environmental contaminants and endocrine disruption to abnormal developmental rate, defects

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TWEEN ® 20