567542-U

Discovery^® HS F5 (3 µm) HPLC Columns

Name: Discovery<SUP>&#174;</SUP> HS F5 (3 µm) HPLC Columns
Brand: SUPELCO

L × I.D. 15 cm × 3 mm, HPLC Column

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UNSPSC Code:

41115700

eCl@ss:

32110501

NACRES:

SB.52

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產品名稱

Discovery ^® HS F5 HPLC 色谱柱, 3 μm particle size, L × I.D. 15 cm × 3 mm, pkg of 1 ea

material

stainless steel column

agency

suitable for USP L43

product line

Discovery^®

feature

endcapped

packaging

pkg of 1 ea

manufacturer/tradename

Discovery^®

extent of labeling

12% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 3 mm

surface area

300 m²/g

surface coverage

4 μmol/m²

impurities

<10 ppm metals

matrix

silica gel, high purity, spherical particle platform
fully porous particle

matrix active group

PFP (pentafluorophenyl) phase

particle size

3 μm

pore size

120 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

Quality Level

100

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本產品	567531-U	567530-U	567501-U
Supelco 567542-U Discovery ^® HS F5 HPLC 色谱柱	Supelco 567531-U Discovery ^® HS F5 HPLC 色谱柱	Supelco 567530-U Discovery ^® HS F5 HPLC 色谱柱	Supelco 567501-U Discovery ^® HS F5 HPLC 色谱柱
particle size 3 μm	particle size 3 μm	particle size 3 μm	particle size 3 μm
separation technique reversed phase	separation technique reversed phase	separation technique reversed phase	separation technique reversed phase
L × I.D. 15 cm × 3 mm	L × I.D. 10 cm × 4 mm	L × I.D. 5 cm × 4 mm	L × I.D. 3.3 cm × 2.1 mm
matrix active group PFP (pentafluorophenyl) phase	matrix active group PFP (pentafluorophenyl) phase	matrix active group PFP (pentafluorophenyl) phase	matrix active group PFP (pentafluorophenyl) phase
pore size 120 Å	pore size 120 Å	pore size 120 Å	pore size 120 Å
agency suitable for USP L43	agency suitable for USP L43	agency suitable for USP L43	agency suitable for USP L43

Features and Benefits

独特的选择性
保留能力与 C18 柱类似（有时需要洗脱能力更强的流动相）
峰形极佳
稳定性好、低流失，适用于 LC-MS 分析
粒径范围从 3μm 到 10μm 可供选择

General description

将 C18 柱方法转换至 Discovery® HS F5 柱的指导原则：
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log P_o/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。

Discovery® HS F5 键合相可提供与 C18 色谱柱截然不同的反相分离。由于 F5 色谱柱上各种化合物通常会在与 C18 相同的时间范围内洗脱出来，使得大部分的 C18 方法可轻松转换到 F5 上。

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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High-performance liquid chromatographic method with UV photodiode-array, fluorescence and mass spectrometric detection for simultaneous determination of galantamine and its phase I metabolites in biological samples.

Jana Maláková et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 853(1-2), 265-274 (2007-04-10)

Galantamine, an alkaloid isolated from the bulbs and flowers of Caucasian snowdrop (Galanthus woronowii, Amaryllidaceae) and related species, is employed in human medicine for the treatment of various neuromuscular and neurodegenerative diseases. After the administration, the products of oxidative biotransformation

HPLC method development, validation, and impurity characterization of a potent antitumor indenoisoquinoline, LMP776 (NSC 725776).

Jennie Wang et al.

Journal of pharmaceutical and biomedical analysis, 124, 267-273 (2016-03-13)

An HPLC method for the assay of a DNA topoisomerase inhibitor, LMP776 (NSC 725776), has been developed and validated. The stress testing of LMP776 was carried out in accordance with International Conference on Harmonization (ICH) guidelines Q1A (R2) under acidic

"Heart-cut" bidimensional achiral-chiral liquid chromatography applied to the evaluation of stereoselective metabolism, in vivo biological activity and brain response to chiral drug candidates targeting the central nervous system.

Umberto M Battisti et al.

Journal of chromatography. A, 1443, 152-161 (2016-03-30)

A "heart-cut" two-dimensional achiral-chiral liquid chromatography triple-quadrupole mass spectrometry method (LC-LC-MS/MS) was developed and coupled to in vivo cerebral microdialysis to evaluate the brain response to the chiral compound (±)-7-chloro-5-(3-furanyl)-3-methyl-3,4-dihydro-2H-1,2,4-benzothiadiazine-1,1-dioxide ((±)-1), a potent positive allosteric modulator (PAM) of AMPA receptor.

Improved separation of furocoumarins of essential oils by supercritical fluid chromatography.

C Desmortreux et al.

Journal of chromatography. A, 1216(42), 7088-7095 (2009-09-15)

Separation of furocoumarins has become of a great interest for cosmetic industry and human health, since the recent directive of the European Union. Furocoumarins are a class of compounds presenting varied substituents linked mainly in two positions to an identical

Advantages of pentafluorophenylpropyl stationary phase over conventional C18 stationary phase--application to analysis of triamcinolone acetonide.

Lucie Havlíková et al.

Talanta, 76(3), 597-601 (2008-07-01)

A pentafluorophenylpropyl (PFPP) stationary phase was for the first time tested for the simultaneous determination of triamcinolone acetonide, its degradation product triamcinolone and two preservatives, methylparaben, and propylparaben. A new simple isocratic reversed phase HPLC method with UV detection, using

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Discovery® HS F5 (3 µm) HPLC Columns