活細胞成像試劑

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A431 epidermal carcinoma cells (85090402) were incubated 16 hours overnight with 3µM BioTracker 520 Green Hypoxia Dye (SCT033) in complete DMEM by gravity perfusion at 37°C under normoxic conditions (20% O2) using the CellASIC® ONIX2 System. After overnight incubation, cells were perfused with complete DMEM media without dye at 1 psi under hypoxic conditions (0% O2) for 16 hours at 37°C. Fluorescent images were captured over 16 hours using 465 nm excitation, and a 525 nm emission filter. The BioTracker 520 Green Hypoxia Dye increases in fluorescence intensity with decreasing oxygen levels (increased hypoxic conditions).

Live Cell Imaging of p62 using LentiBrite™ Fluorescent Biosensors

Human mesenchymal stem cells (MSCs), were plated in a chamber slide and transduced with LentiBrite™ RFP-p62 lentiviral particles at an MOI of 40 for 24 hours. After media replacement and 24 hours further incubation, cells were incubated for 4 hours in EBSS containing a lysosome inhibitor, to induce autophagy and inhibit lysosomal degradation of autophagosomes.

Live Cell Imaging of β-Actin Cytoskeleton Proteins using LentiBrite™ Fluorescent Biosensors

U2OS cells were plated in a chamber slide and transduced with LentiBrite™ GFP-β-actin lentiviral particles at an MOI of 20 for 24 hours. After media replacement and an additional 48 hours of further incubation, the cells were treated with 2 µM cytochalasin-D for another 2 hours, to induce actin depolymerization. The GFP- β -actin displays a filamentous distribution in the untreated cells, and upon actin depolymerization, adopts a punctate distribution. LentiBrite™ Lentiviral Biosensors are a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.

Live Cell Imaging of Autophagy using LentiBrite™ Fluorescent Biosensors

HT-1080 cells were plated in a chamber slide and transduced with LentiBrite™ GFP-LC3 lentiviral particles at an MOI of 20 for 24 hours. After media replacement and 48 hours further incubation, cells were incubated for 4 hours in EBSS containing a lysosome inhibitor, to induce autophagy and inhibit lysosomal degradation of autophagosomes. LentiBrite™ Lentiviral Biosensors are a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.

Live Cell Imaging of Apoptosis in Immune Cells using the CellASIC® ONIX2 Microfluidic System

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

Fluorescent cell tracking dyes, in combination with flow and image cytometry, are powerful tools with which to study the interactions and fates of different cell types in vitro and in vivo.

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活細胞成像試劑

PKH 與 CellVue^® 螢光細胞連結劑標記

PKH與CellVue®染料的主要特性包括：

LentiBrite™ 螢光慢病毒生物感測器

慢病毒生物感測器特點：

BioTracker^® 活細胞螢光染料與染色劑

LuminiCell Tracker™ 螢光奈米粒子

相關資源

活細胞成像試劑

產品

PKH 與 CellVue® 螢光細胞連結劑標記

PKH與CellVue®染料的主要特性包括：

LentiBrite™ 螢光慢病毒生物感測器

慢病毒生物感測器特點：

BioTracker® 活細胞螢光染料與染色劑

LuminiCell Tracker™ 螢光奈米粒子

相關資源

相關影片

PKH 與 CellVue^® 螢光細胞連結劑標記

BioTracker^® 活細胞螢光染料與染色劑