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Merck
en首頁支援計算機與應用程式去垢劑 Western Blot 剝離緩衝液

去垢劑 Western Blot 剝離緩衝液

對 Western 印跡進行剝離和重新印跡可保存樣品，並快速修正或調整抗體濃度和孵育時間。

本配方是使用表面活性劑十二烷基硫酸鈉（SDS）和還原劑β-巯基乙醇（BME）來去除抗體和化學發光或螢光檢測試劑的 Western 印迹剝離溶液。

本計算器可準確製備任何毫升容量的去垢劑 Western 印迹剝離溶液。輸入您所需的容量，點擊計算按鈕，表格中就會跳出所需的各成分用量。

洗滌劑型 Western 印迹剝離液配方計算機

100 mL Recipe Desired Volume: mL    
20 mL of 10% SDS (71736)   mL of 10% SDS
12.5 mL of 0.5 M Tris-HCl pH 6.8 (FW 157.60)    mL of 0.5 M Tris HCl pH 6.8
0.8 mL of β-mercaptoethanol (63689)   mL of β-mercaptoethanol
Adjust final volume to 100 mL with Milli-Q® Water Adjust final volume to   mL with Milli-Q® Water
  Procedure
 
  1. In a fume hood, place the blot in detergent stripping buffer and incubate with agitation for 30 minutes at 50 °C.
  2. Place the blot in 1X PBS and agitate for 10 minutes. Repeat with fresh buffer.
  3. Optional: Repeat the initial detection protocol (omitting the primary antibody step) to make sure that the antibodies have been inactivated or removed from the membrane.
  4. Place the blot in fresh 1X PBS and agitate for 10 minutes.
  5. Proceed to the blocking step for the next round of immunodetection.
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