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Effect of multi-frequency power ultrasound (MFPU) treatment on enzyme hydrolysis of casein.

Ultrasonics sonochemistry (2020-01-17)
Baoguo Xu, Jun Yuan, Lin Wang, Feng Lu, Benxi Wei, Roknul S M Azam, Xiaofeng Ren, Cunshan Zhou, Haile Ma, Bhesh Bhandari
ABSTRACT

Effect of multi-frequency power ultrasound (MFPU) pretreatments on the degree of hydrolysis (DH) and mechanism of casein during alcalase enzymolysis was investigated. Results showed that MFPU pretreatment in tri-frequency 20/40/60 kHz mode significantly (p < 0.05) improved the DH value of casein. Variation of intrinsic fluorescence spectrum indicated the unfolding and degradation of casein occurred after MFPU pretreatment. Fourier transform infrared spectra showed that α-helix and β-sheet content of MFPU pretreated casein decreased, while β-turn and random coil content increased. Surface topography and nanostructures of caseins were found modified after MFPU pretreatments by the analysis of scanning electron microscopy (SEM) and atomic force microscopy (AFM). The SEM analysis also indicated that the enzymolysis residues of casein pretreated by MFPU were smaller than untreated samples. In conclusion, the MFPU can be used as an efficient pretreatment method to promote the enzymolysis of casein.

MATERIALS
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Product Description

Sigma-Aldrich
Casein from bovine milk, technical grade