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Volatile Profile Characterization of Croatian Commercial Sparkling Wines.

Molecules (Basel, Switzerland) (2020-09-26)
Ana-Marija Jagatić Korenika, Darko Preiner, Ivana Tomaz, Ana Jeromel
ABSTRACT

Commercial sparkling wine production represents a relatively low but important part of the Croatian wine production, especially in the Zagreb county. This study presents the results of volatile aroma compounds profile and organic acid composition of commercial sparkling wine samples from three vine-growing regions in Zagreb county. In total, 174 volatile aroma compounds were identified, separated between their chemical classes (aldehydes, higher alcohols, volatile phenols, terpenes, C13-norisoprenoids, lactones, esters, fatty acids, sulfur compounds, other compounds, other alcohols). Higher alcohols such as phenylethyl and isoamyl alcohol as well as 2-methyl-1-butanol, and esters such as diethyl succinate, ethyl hydrogensuccinate, and ethyl lactate had the strongest impact on the volatile compounds profile of Zagreb county sparkling wine. The presence of diethyl glutarate and diethyl malonate, compounds whose concentrations are influenced by yeast autolysis or caused by chemical esterification during the ageing process, was also noted. The influence of every single volatile aroma compound was evaluated by discriminant analysis using forward stepwise model. The volatile profiles of traditional sparkling wines from Croatia were presented for the first time. It is hoped the results will contribute to better understanding the quality potential and to evaluate possible differences on the bases of detected aroma concentrations and multivariate analysis.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Phosphate Buffer, powder
Sigma-Aldrich
4-Methyl-1-pentanol, 97%
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N-Ethylacetamide, 99%
Sigma-Aldrich
2,4-Heptadienal, technical grade, 90%
Sigma-Aldrich
Ethyl 3-hydroxyhexanoate, ≥98%, FG
Sigma-Aldrich
Linalyl formate, FCC
Sigma-Aldrich
α-Naphthyl Butyrate solution
Sigma-Aldrich
Methyl geranate, AldrichCPR
Sigma-Aldrich
Diethyl glutarate, ≥99%
Sigma-Aldrich
3-Ethoxy-1-propanol, 97%
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Homovanillyl alcohol, 99%
Sigma-Aldrich
CD117, c-kit Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections)
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CD31 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections)
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CD4 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections)
Sigma-Aldrich
Methyl 2-hydroxy-3-methylbutanoate
Sigma-Aldrich
CD10 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections)
Sigma-Aldrich
CD15 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections)
Sigma-Aldrich
CD1a Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections)
Sigma-Aldrich
CD21 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections)
Sigma-Aldrich
CD3 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections)
Sigma-Aldrich
CD38 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections)
Sigma-Aldrich
CD43 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections)
Sigma-Aldrich
CD57 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections)
Sigma-Aldrich
CD7 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections)
Sigma-Aldrich
4-Ethylcyclohexanol, AldrichCPR