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Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.

Nucleic acids research (1977-01-01)
J Casey, N Davidson
PMID19730
ABSTRACT

The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DNA:DNA rates are negligible and RNA:DNA rates close to optimal.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Formamide, ReagentPlus®, ≥99.0% (GC)
Sigma-Aldrich
Formamide, BioUltra, for molecular biology, ≥99.5% (T)
Millipore
OmniPur® Formamide, Deionized
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Formamide, puriss. p.a., ACS reagent, ≥99.5% (GC/T)
Sigma-Aldrich
Formamide, ACS reagent, ≥99.5%
Supelco
Formamide, for analysis EMSURE®
Millipore
Formamide, Molecular Biology Grade - CAS 75-12-7 - Calbiochem, Deionized. Adenine deaminase inhibitor. Used in DNA stripping during genetic fingerprinting techniques.
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Formamide, EMPLURA®
Sigma-Aldrich
Formamide, spectrophotometric grade, ≥99%