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  • Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.

Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.

Nucleic acids research (1977-01-01)
J Casey, N Davidson
PMID19730
ABSTRACT

The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DNA:DNA rates are negligible and RNA:DNA rates close to optimal.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Formamide, spectrophotometric grade, ≥99%
Sigma-Aldrich
Formamide, ACS reagent, ≥99.5%