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Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor.

Journal of the American Chemical Society (2008-09-03)
Masayasu Taki, Mika Desaki, Akio Ojida, Shohei Iyoshi, Tasuku Hirayama, Itaru Hamachi, Yukio Yamamoto
ABSTRACT

We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free and Cd2+-bound forms, respectively, and has a dissociation constant of 0.16 nM for Cd2+. CadMQ is cell permeable and locates within the acidic compartments of the cells. We further show that CadMQ is a useful tool to ratiometrically probe the change in the intracellular Cd2+ levels with the use of two excited wavelengths.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
7-Amino-4-methylcoumarin, 99%
Sigma-Aldrich
7-Amino-4-methylcoumarin, Chromophore for substrates