MilliporeSigma
  • Home
  • Search Results
  • Vortex-assisted liquid-liquid microextraction coupled with derivatization for the fluorometric determination of aliphatic amines.

Vortex-assisted liquid-liquid microextraction coupled with derivatization for the fluorometric determination of aliphatic amines.

Journal of chromatography. A (2012-06-26)
Wei-Yao Chang, Chin-Yi Wang, Jeng-Lyan Jan, Yu-Shiu Lo, Chien-Hou Wu
ABSTRACT

A new one-step derivatization and microextraction technique was developed for the fluorometric determination of C(1)-C(8) linear aliphatic primary amines in complex sample solutions containing high levels of amino acids. In this method, amines were derivatized with o-phthalaldehyde (OPA) and 2-mercaptoethanol (2-ME) in aqueous solution and extracted simultaneously by vortex-assisted liquid-liquid microextraction (VALLME). Parameters affecting the extraction efficiency were investigated in detail. The optimum conditions were as follows: 50 μL of isooctane as the extractant phase; 2.0 mL aqueous donor samples with 12 mM OPA, 24 mM 2-ME, and 0.1 M borate buffer at pH 10; 1 min vortex extraction time; centrifugation for 4 min at 6000 rpm. After centrifugation, the enriched analytes in the floated extractant phase were determined by HPLC-FL in less than 14 min. Under the optimum conditions, the limits of detection were of the order of 0.09-0.31 nM. The calibration curves showed good linearity over the investigated concentration range between 0.4 and 40 nM. The proposed method has been applied to the determination of aliphatic amines in acidophilus milk, beer, and Cu(II)/amino acid solution.

MATERIALS
Product Number
Brand
Product Description

Supelco
2,2,4-Trimethylpentane, analytical standard
Sigma-Aldrich
Phthaldialdehyde Reagent, Solution Incomplete
Sigma-Aldrich
Phthaldialdehyde Reagent, Solution Complete
Sigma-Aldrich
2,2,4-Trimethylpentane, anhydrous, 99.8%
Sigma-Aldrich
2,2,4-Trimethylpentane, puriss. p.a., ≥99.5% (GC)
Sigma-Aldrich
2,2,4-Trimethylpentane, ReagentPlus®, ≥99%
Sigma-Aldrich
2,2,4-Trimethylpentane, puriss. p.a., ACS reagent, ≥99.5% (GC)
Sigma-Aldrich
2,2,4-Trimethylpentane, ACS reagent, ≥99.0%
Sigma-Aldrich
2,2,4-Trimethylpentane, suitable for HPLC, ≥99%
Sigma-Aldrich
2,2,4-Trimethylpentane, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.5%
Sigma-Aldrich
Phthaldialdehyde, suitable for HPLC fluorimetric detection of amino acids, ≥99% (HPLC), powder
Sigma-Aldrich
Phthaldialdehyde, ≥97% (HPLC), powder (may contain lumps)
Sigma-Aldrich
Phthaldialdehyde, for fluorescence, ≥99.0% (HPLC)
Supelco
Density Standard 692 kg/m3, H&D Fitzgerald Ltd. Quality