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Efficacy of all-trans retinoid acid in preventing nickel induced cardiotoxicity in myocardial cells of rats.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (2012-09-20)
Siyue Lou, Like Zhong, Xiaochun Yang, Tao Xue, Renhua Gai, Difeng Zhu, Yuqin Zhao, Bo Yang, Meidan Ying, Qiaojun He
ABSTRACT

Nickel, a metal commonly found in battery plants and welding factories, has potential cardiotoxicity, while all-trans retinoid acid (atRA) can promote cardiovascular repair and myocardial recovery. The purpose of this study was to investigate whether atRA could prevent cardiotoxicity induced by nickel both in vitro and in vivo. In the study, a rat myocardial cell line (H9c2) exposed to different concentrations of nickel chloride (NiCl(2)) displayed apoptotic features accompanied by reactive oxygen species generation. In addition, NiCl(2) also caused obvious apoptosis and systolic dysfunction in primary myocardial cells. Treatment with atRA efficiently attenuated the cytotoxicities triggered by NiCl(2) as it significantly mitigated ROS generation and decreased MAP kinases activity in NiCl(2)-treated cardiomyocytes. Additionally, NiCl(2) exposure caused obvious arrhythmia in Sprague-Dawley rats with the maximum tolerance dose of NiCl(2) between 2 and 3mg/kg. A combinational intragastric administration of 40mg/kg atRA can partially reverse NiCl(2)-induced arrhythmia in rats. Our results suggested that atRA might have therapeutic potential in alleviating the adverse effects of nickel on the cardiovascular system.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Nickel(II) chloride, 98%
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Nickel(II) chloride hexahydrate, 99.9% trace metals basis
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Nickel(II) chloride hexahydrate, ReagentPlus®
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Nickel(II) chloride hexahydrate, BioReagent, suitable for cell culture
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Nickel(II) chloride, anhydrous, powder, 99.99% trace metals basis
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Nickel(II) chloride hexahydrate, puriss. p.a., ≥98%
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Nickel(II) chloride hexahydrate, 99.999% trace metals basis
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Nickel(II) chloride hydrate, 99.95% trace metals basis