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Lipid droplet synthesis is limited by acetate availability in starchless mutant of Chlamydomonas reinhardtii.

FEBS letters (2013-01-15)
Rishiram Ramanan, Byung-Hyuk Kim, Dae-Hyun Cho, So-Ra Ko, Hee-Mock Oh, Hee-Sik Kim
ABSTRACT

Phenotypic and genotypic changes in Chlamydomonas reinhardtii BafJ5, a starchless mutant, with respect to lipid metabolism was studied in different trophic states under nitrogen (N) sufficient and limited conditions. Interestingly, cellular lipid content increased linearly with input acetate concentration with highest lipid content (∼42%) under nitrogen limitation and mixotrophic state. RT-qPCR studies indicate that key fatty acid biosynthesis genes are down-regulated under N limitation but not under mixotrophic state, whereas, ACS2, encoding Acetyl-CoA synthetase, and DGTT4, encoding Diacylglycerol O-acyltransferase, are up-regulated under all conditions. These results collectively indicate that acetate is the limiting factor and central molecule in lipid droplet synthesis. The study also provides further evidence of the presence of a chloroplast pathway for triacylglycerol synthesis in microalgae.

MATERIALS
Product Number
Brand
Product Description

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