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Protease inhibitor domain encoded by an amyloid protein precursor mRNA associated with Alzheimer's disease.

Nature (1988-02-11)
R E Tanzi, A I McClatchey, E D Lamperti, L Villa-Komaroff, J F Gusella, R L Neve
ABSTRACT

Amyloid B-protein/amyloid A4 is a peptide present in the neuritic plaques, neurofibrillary tangles and cerebrovascular deposits in patients with Alzheimer's disease and Down's syndrome (trisomy 21) and may be involved in the pathogenesis of Alzheimer's disease. Recent molecular genetic studies have indicated that amyloid protein is encoded as part of a larger protein by a gene on human chromosome 21 (refs 6-9). The amyloid protein precursor (APP) gene is expressed in brain and in several peripheral tissues, but the specific biochemical events leading to deposition of amyloid are not known. We have now screened complementary DNA libraries constructed from peripheral tissues to determine whether the messenger RNA encoding APP in these tissues is identical to that expressed in brain, and we identify a second APP mRNA that encodes an additional internal domain with a sequence characteristic of a Kunitz-type serine protease inhibitor. The alternative APP mRNA is present in both brain and peripheral tissues of normal individuals and those with Alzheimer's disease, but its pattern of expression differs from that of the previously reported APP mRNA.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Amyloid Precursor Protein, C-Terminal antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-β-Amyloid antibody produced in mouse, clone BAM-10, ascites fluid
Sigma-Aldrich
Anti-Amyloid Precursor Protein, N-Terminal antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
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Anti-APP Antibody, APP 643-695 CT fragment, clone 2.F2.19B4, ascites fluid, clone 2.F2.19B4, Chemicon®
Sigma-Aldrich
Anti-phospho-APP (pThr668) antibody produced in rabbit, affinity isolated antibody, aqueous glycerol solution, 10 blots
Sigma-Aldrich
Anti-Amyloid Precursor Protein, KPI Domain antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

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