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Characterization of a human reference standard for antibody to Plasmodium falciparum merozoite surface protein 1(42).

The American journal of tropical medicine and hygiene (2005-06-21)
In-Kyu Yoon, Evelina Angov, David Larson, D Gray Heppner, James F Cummings, V Ann Stewart
ABSTRACT

Volunteers vaccinated with a candidate malaria vaccine containing merozoite surface protein 1(42) (MSP-1(42)) exhibit antibodies to MSP-1(42) that are measured by enzyme-linked immunosorbent assay (ELISA). The purpose of this study was to make a human reference standard for MSP-1(42) antibody measured in absolute quantity units using pooled plasma samples known to contain high titers of MSP-1(42) antibody based on previous ELISA results. Immobilized metal affinity chromatography was used to determine the amount of MSP-1(42) antibody in this plasma pool. Hexahistidine-tagged MSP-1(42) antigen adsorbed to nickel-chelating resin was used to capture MSP-1(42) antibody from the plasma pool. The intact MSP-1(42) antibody-antigen complexes were eluted and total IgG was measured by an ELISA standardized against purified human IgG. In this way, the human reference standard was determined to contain 48.3 microg/mL of MSP-1(42) antibody. This reference standard may be useful as a quantitative working standard for measuring MSP-1(42) antibody response in future vaccine clinical trials involving MSP-1.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
IgG from human serum, reagent grade, ≥95% (HPLC), buffered aqueous solution

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