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  • A new reagent which may be used to introduce sulfhydryl groups into proteins, and its use in the preparation of conjugates for immunoassay.

A new reagent which may be used to introduce sulfhydryl groups into proteins, and its use in the preparation of conjugates for immunoassay.

Analytical biochemistry (1983-07-01)
R J Duncan, P D Weston, R Wrigglesworth
ABSTRACT

A synthesis of the N-hydroxysuccinimide ester of S-acetylthioacetic acid is described. This material is stable when stored dry and has advantages over the currently available reagents used to introduce sulfhydryl groups into a variety of proteins. Proteins modified with this reagent can be used to prepare conjugates for enzyme immunoassay. The conjugation techniques described cause little or no loss of either enzyme activity or antibody titer and function, and the conjugates contain little polymeric material.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Streptavidin−Maleimide from Streptomyces avidinii
Sigma-Aldrich
Peroxidase, Maleimide Activated from horseradish, lyophilized powder, >200 units/mg protein
Sigma-Aldrich
S-Acetylthioglycolic acid N-hydroxysuccinimide ester, ≥95% (TLC), powder