HPLC Analysis of Barbiturates on Ascentis® C18
Materials
related product
Product No.
Description
Pricing
Secobarbital solution
1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Phenobarbital solution
1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Butabarbital solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Barbiturate Mix-5 solution
250 μg/mL each component in methanol, ampule of 1.0 mL, certified reference material, Cerilliant®Pentobarbital solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
mobile phase
[A] methanol; [B] water (50:50, A:B)
flow rate
1.0 mL/min
column temp.
35 °C
detector
UV, 214 nm
injection
10 μL
sample
as indicated in 65:35 water:methanol
Description
Analysis Note
Barbiturates are commonly abused and among the most widely tested compounds in clinical, forensic, or therapeutic drug monitoring applications. Shown here is the baseline separation of a set of barbiturates on an Ascentis C18 column. Highest grade HPLC solvents were used to supply low background interference and low particulate contamination for robust, trouble-free operation. Cerilliant and Sigma-Aldrich reference standards provided reliable identification and quantification.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany