The Scepter 3.0 sensors are single use only.
PHCC360KIT
Contador celular automático que cabe en la palma de la mano Scepter 3.0
Rapid cell counts in a handheld easy to use format, includes pkg of 60 μm Scepter 3.0 Cell Counter Sensors
Scepter 3.0 Handheld Automated Cell Counter
Sinónimos:
Cell counting instrument
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1 EA
$4,480.00
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UNSPSC Code:
41131501
NACRES:
NC.56
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Servicio técnico
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Permítanos ayudarlemanufacturer/tradename
Scepter
technique(s)
cell counting: suitable
particle size
6-36 μm
compatibility
for use with cells sized 4.0–25.0 μm
shipped in
ambient
1 of 4
Este artículo | PHCC340KIT | PHCC360500 | PHCC340050 |
|---|---|---|---|
| compatibility for use with cells sized 4.0–25.0 μm | compatibility for use with cells sized 4.0–25.0 μm | compatibility Scepter for use with cells sized 8.0–25.0 μm | compatibility Scepter for use with cells sized 5.0–15.0 μm |
| technique(s) cell counting: suitable | technique(s) cell counting: suitable | technique(s) cell counting: suitable | technique(s) cell counting: suitable |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| manufacturer/tradename Scepter | manufacturer/tradename Scepter | manufacturer/tradename Scepter | manufacturer/tradename Scepter |
| particle size 6-36 μm | particle size 4-18 μm | particle size 6-36 μm | particle size 4-18 μm |
General description
El contador celular automático Scepter 3.0 proporciona recuentos celulares rápidos en un formato fácil de usar que cabe en la palma de la mano. Destinado exclusivamente a investigación, el Scepter 3.0 se ha probado con tipos de células representativos de los que se utilizan actualmente. Después de preparar una dilución del cultivo celular de interés, las instrucciones en pantalla guían al usuario a través del proceso de muestreo. En menos de 30 segundos después de que el contador de células Scepter aspire una muestra en el sensor del contador de células Scepter, la pantalla del contador mostrará:
El contador celular Scepter 3,0 es compatible con los sensores del contador celular Scepter 3.0 de 40 uM y 60 uM. Este kit (PHCC360KIT) contiene un paquete gratuito de 50 sensores de 60 uM.
- Concentración celular
- Tamaño celular promedio
- Volumen celular promedio
- Histograma de distribución por tamaño o volumen
El contador celular Scepter 3,0 es compatible con los sensores del contador celular Scepter 3.0 de 40 uM y 60 uM. Este kit (PHCC360KIT) contiene un paquete gratuito de 50 sensores de 60 uM.
Other Notes
- 1 Contador de células automático que cabe en la palma de la mano Scepter 3.0
- 1 Vial de microesferas problema
- 1 Estación de carga
- 50 Sensores (60μM)
El contador celular Scepter 3.0 no es compatible con sensores de generaciones anteriores; solo es compatible con los sensores del contador celular Scepter 3.0 que se muestran en los productos relacionados.
For use with
Referencia del producto
Descripción
Precios
Clase de almacenamiento
10-13 - German Storage Class 10 to 13
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
The Scepter™ 3.0 Cell Counter, when used in conjunction with a 40 μm aperture sensor, can accurately count and precisely size a wide range of cell types and particles. This application note demonstrates two examples of experiments using the instrument’s sensitive size-discriminating capacity for rapid qualitative assessment of individual cell populations.
Data for validated human cell lines using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.
Learn how Sterivex™ pressure-driven sterile filters can be used for large-volume sample processing in environmental research and testing.
Find answers to frequently asked questions about the Millicell® ERS 3.0, from probe depth adjustment to instrument maintenance and troubleshooting.
Protocolos
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Contenido relacionado
Streamline TEER data capture and analysis with user-friendly enhancements including an intuitive touchscreen interface, standing in-well probe, and automatic data logging.
Scepter™ counting is 7 to 10 times faster than hemocytometry-and also faster than other automated counters. Learn more about how this amazing technology works
A list of which mammalian and other key phenotypes have been validated for Scepter™ counting precision.
Optimice la captura y el análisis de los datos de TEER (resistencia eléctrica transepitelial) con mejoras fáciles de utilizar que abarcan una interfaz de pantalla táctil intuitiva, una sonda independiente para introducir en los pocillos y el registro automático de los datos.
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Can I confirm if the sensors are single-use only please?
1 answer-
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What is the maximum size of cell clumps that can pass through the Scepter 3.0? Also, what size screen is used to break up the cell clump aggregates in both Scepter 2.0 and 3.0?
1 answer-
The maximum size of cell clumps that can pass through the Scepter 3.0 is determined by the aperture limits, which are 40um and 60um for sensors 40 and 60, respectively. Unlike the Scepter 2.0, the Scepter 3.0 does not use a screen to break up cell clump aggregates; instead, it employs a set of pillars to achieve this function. In contrast, the Scepter 2.0 utilizes a 35um Nylon filter to break up cell clump aggregates.
Helpful?
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Is this appropriate for size 15-23 μm cell? Does the Kit include also the charger? How many 60/40 μm sensors are included Which sensors can be used with this instrument? Which sensors are appropriate for cells size 15-23 μm and what is their price?
1 answer-
Please see the answers to the following questions below:
1. Is this appropriate for size 15-23 μm cells?
Yes, the Scepter 3.0 is suitable. As listed on the product detail page in the DESCRIPTION section for use with cells sized 4.0–25.0 μm.2. Does the Kit include the charger?
This product does include 60μM sensors and a charging station.3. How many 60/40 μm sensors are included
As mentioned on the product detail page in the DESCRIPTION section under 'Components', it includes 50 Sensors.4. Which sensors can be used with this instrument?
This product is only compatible with Scepter 3.0 Cell Counter Sensors shown in the Related Products on this page. Please see the link below to review additional information regarding the Scepter 3.0:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-counting-and-health-analysis/scepter-cell-counter5. Which sensors are appropriate for cells size 15-23 μm and what is their price?
See question 1, this product is suitable for the cell size of 15-23 μm.Regarding pricing, Please navigate to the link https://www.sigmaaldrich.com/support/customer-support, and click on "Products Pricing & Availability" under the Products Section with all the required information so that a member of the Customer Service team can reach out to assist further. Thank you.
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Does it require Trypan blue or another dye? Does it distinguish between live and dead cells?
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The Scepter is a particle counter, but it will not differentiate live from dead cells. The device can be used to gauge cell health on the basis of cell size. For example, in cell types where apoptotic cells are smaller in size compared to non-apoptotic cells. This shift in size can be quantified using the device option "Edit Histogram" to either gate around this population or exclude the population from the count.
Please see the following manual below for more information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/799/484/00110230w-rev1020-ms.pdfHelpful?
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