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95290

Methyl Blue

suitable for microscopy

Synonym(s):

Acid blue 93, Aniline blue water soluble, Cotton blue, Poirriers blue, Water blue

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Size/SKUAvailabilityPrice
5 g

Available to ship TODAY

$34.70
25 g

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$114.00
100 g

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$362.00

About This Item

Empirical Formula (Hill Notation):
C37H27N3Na2O9S3
CAS Number:
Molecular Weight:
799.80
UNSPSC Code:
12171500
NACRES:
MA.02
PubChem Substance ID:
EC Number:
249-352-9
Colour Index Number:
42780
MDL number:

$34.70


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form

powder or crystals

Quality Segment

technique(s)

microbe id | staining: suitable

pH

9.4-14

solubility

water: 0.01 g/10 mL, blue to very deep blue

εmax

≥200 at 310-320 nm, ≥400 at 595-605 nm

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].OS(=O)(=O)c1ccc(Nc2ccc(cc2)\C(c3ccc(Nc4ccc(cc4)S([O-])(=O)=O)cc3)=C5/C=CC(\C=C5)=N/c6ccc(cc6)S([O-])(=O)=O)cc1

InChI

1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2

InChI key

MCPLVIGCWWTHFH-UHFFFAOYSA-L

General description

Methyl Blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.

Application

Methyl Blue has been used:
  • to stain leaf disks for callose visualization within the cells
  • to incubate cells for 4′,6-diamidino-2-phenylindole (DAPI)/septa staining
  • as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin
  • in Mann′s stain as it involves a methyl blue-eosin mixture

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This Item
M5528M69001150207
description

suitable for microscopy

description

certified by the BSC

description

powder or crystals

description

United States Pharmacopeia (USP) Reference Standard

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

-

technique(s)

-

suitability

suitable for microscopy

suitability

-

suitability

-

suitability

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

pharmaceutical (small molecule)

form

powder or crystals

form

powder

form

powder or crystals

form

-

solubility

water: 0.01 g/10 mL, blue to very deep blue

solubility

H2O: 1 mg/mL, blue

solubility

H2O: 1 mg/mL, blue

solubility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-


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Storage Class

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

>572.0 °F - Pensky-Martens closed cup

Flash Point (°C)

> 300 °C - Pensky-Martens closed cup

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Gloves



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