MTOX1095

MRP5 Knockout Caco-2 Cells

human male colorectal tissue (Source Disease: colon adenocarcinoma)

• UNSPSC Code: 41106514
• NACRES: NA.81
• Biological source: human male colorectal tissue (Source Disease: colon adenocarcinoma )

Properties

• Product Name: MRP5 Knockout Caco-2 Cells, one vial
• biological source: human male colorectal tissue (Source Disease: colon adenocarcinoma )
• Quality Segment: 200
• form: liquid
• technique(s): drug transporter assay: suitable, permeability assay: suitable
• application(s): ADME/TOX
• storage temp.: −196°C
• Gene Information: human ... ABCC5(10057)

Description

General description

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MRP5 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCC5 (MRP5) efflux transporter.

Application

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Features and Benefits

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:

• A functional knockout of the MRP5 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP5 substrate
• The vial format enables the MRP5 knockout cells to be included in standard drug transporter protocols
• Human assay with no interference from animal inhibitors
• Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

Legal Information

ADME/Tox Cell Lines License

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

Safety Information

• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• Storage Class: 12 - Non Combustible Liquids